KT对刺葡萄愈伤组织花青素含量的影响

Effect of KT on anthocyanin content in callus of brier grape (Vitis davidii Foёx)

摘要: 以长期保持的紫红色刺葡萄愈伤组织DLR细胞系为试验材料，研究不同KT浓度对其花青素和原花青素含量的影响，结果表明：KT可以有效调控刺葡萄愈伤组织花青素和原花青素的合成，其中以MS+2，4-D 1.0 mg/L+KT 0.05 mg/L培养基效果最佳，花青素和原花青素含量分别达到66.95 μg/g（FW）和3947.50 μg/g（FW），分别是对照的2.44倍和1.68倍。

Abstract: Using the long-term maintained and purplish red callus of brier grape as test material, the effect of different KT concentration on its anthocyanin and proanthocyanidinscontent was investigated in this paper. The result showed that the anthocyanin and proanthocyanidins synthesis of brier grape callus could be regulated effectively by KT. The optimal medium was MS+2,4-D 1.0 mg/L+KT 0.05 mg/L, the anthocyanincontent and proanthocyanidinscontent were up to 66.95 μg/g(FW) and 3947.50 μg/g(FW), and they were 2.44 times and 1.68 times as control respectively.