Abstract: To elucidate the regulatory mechanisms of Dictyophora indusiata cultivation on soil phosphorus（P）cycling in paddy fields, this study employed D. indusiata cultivation plots（JXZS）and adjacent control plots（JXCK）as experimental subjects. We systematically investigated the multi-scale impacts of D. indusiata cultivation on P cycling through the determination of soil P fractions, activities of key P-cycling enzymes, and functional metagenomic analysis. The results demonstrated that:（1）D. indusiata cultivation significantly increased soil total phosphorus（TP）content by 54.7% and available phosphorus（AP）content by 137.5%, concurrently activating acid phosphatase（ACP）activity by 33.0%. Phytase（PHY）activity exhibited a significant increase, whereas phosphodiesterase（PDE）activity remained unchanged;（2）The microbial P metabolic strategy shifted from a "low-phosphorus adaptation" phenotype towards a "high-efficiency utilization" phenotype. Control plots exhibited significant enrichment of genes associated with phosphorus storage（ppk2）and recalcitrant organic P utilization（phnP）, alongside enzyme systems including EC 3.1.4.55（complex organic P degradation）. In contrast, cultivation plots showed significant enrichment of the E3.1.3.8 gene（labile organic P mineralization）and its corresponding enzymatic system;（3）Soil enzyme activities（PHY, PDE）emerged as key drivers of microbial functional variation, displaying significant negative correlations with core functional genes（ppx-gppA, ppa）. Total P content exhibited no significant regulatory effect on microbial functionality;（4）The P metabolic network exhibited a "loose structure with core node-driven" topology. Within the gene network, only two connector nodes（phnO, E3.1.3.1/phoA/phoB）were identified. E3.1.3.8 and phoN functioned as core genes within the bipartite network, while the majority of microbial genera constituted peripheral nodes. In conclusion, Dictyophora indusiata cultivation systematically reshapes the phosphorus metabolic functional pattern of soil microorganisms by increasing the soil phosphorus pool capacity and activating key phosphatases in farmland. This process drives microorganisms to shift their strategy from relying on endogenous phosphorus reserves to prioritizing the utilization of exogenous labile organic phosphorus, and the optimization of this metabolic strategy is an important microbiological mechanism for the improvement of soil phosphorus cycling efficiency in D. indusiata cultivation plots.