Abstract: Enhancing the bactericidal effectiveness of traditional antibiotics is a key approach to tackling the increasing problem of bacterial resistance. In this study, the antibacterial effects of exogenous coenzyme Q0（CoQ0）combined with gentamicin against Pseudomonas aeruginosa were tested through bactericidal assays. The results show that CoQ0 alone has no direct antimicrobial activity against Pseudomonas aeruginosa; however, at a concentration of 600 μg·mL⁻¹, it significantly boosts the bactericidal activity of gentamicin against stationary-phase bacteria. This boosting effect depends on both concentration and time. Additionally, CoQ0 specifically increases the killing efficiency of other aminoglycoside antibiotics, such as tobramycin, but does not show synergistic effects with β-lactam antibiotics. The potential for synergy was further confirmed across eight clinically isolated drug-resistant strains. These findings support the idea of using CoQ0 as an adjuvant in antibiotic treatment to fight Pseudomonas aeruginosa resistance.