Abstract: In order to explore the epidemiology of antibiotics resistance carried by pet dogs' intestinal microorganisms and explain the relationship between the microbial resistance in raising animals and the resistance genes of human pathogenic microorganism, the antibiotic resistance carried by the culturable enterobacter in the intestinal tract of pet dogs was investigated, as well as the species, diversity and ecological distribution, by using the bacterial culture, isolation and identification and the replica plating technology. The results showed that the culturable bacteria in the intestinal tract of pet dogs had a high proportion of drug resistance and multi-drug resistance. Among which, Escherichia coli had a higher drug resistance rate to the drugs that were used earlier such as ampicillin, sulfonamides, chloramphenicol, streptomycin and tetracycline, while was more sensitive to cephalosporins with a short clinical time. Almost all of the bacteria surveyed had at least one antibiotic resistance, and more than half of the isolatable intestinal bacteria had at least five antibiotic resistance. In summary, the occurrence of intestinal microbial resistance was closely related to the usage time of antibiotics, and the wide distribution of the resistance of the culturable bacteria in the intestinal tract of pet dogs indicated that pets were likely to be the potential route for the transmission of resistance genes between the environment and human pathogenic bacteria.