Abstract: Comparison test on mycelium activity and mixed fungi-resistant capacity of ten different strains of Pleurotus eryngii were conducted, the results showed that the strains Xingle, Xing A, Ple0028, Ple0038 and Ple0045 showed higher mycelium activity and mixed fungi-resistant capacity in mycelium growing stage, which could be used in further experiments on mushroom fructification.