Abstract: Based on production practice, three aspects of expanding propagation production of gerbera were studied. Experimental results showed that in the multiplication culture stage, interchangeably using two hormones KT and BA or combine using KT and BA could effectively avoid weaken and vitrification of seedlings at too early stage caused by improper use of hormone, and could make large quantities of intermediate propagule stable proliferation during the subculture process. The best practices of subculture multiplication of gerbera was cutting operations to “remove leaves and remain small pieces of 1/2 basal callus clump buds”, and without lighting can effectively save about 15% of light energy in the first 3 d after transgenerational transfer. Lower cytokinin and increase dosage of auxin could achieve the effect of the synchronous seedling and proliferation and was beneficial to separate cutting to improve work efficiency and rooting culture, and the suitable medium for rooting culture was 1/2MS+IAA0.5 mg/L+IBA0.5 mg/L+AC1.0 g/L, and during transplanting culture, the technology of moisture seedling cultivation was used.