Abstract: A comparison test of six middle-season ratoon rice varieties harvest by machine was carried out by Pucheng Agricultural Technique Extension Station. Their growth period, yield in both main and ratooning seasons, economic traits and regenerative capacity were comprehensively analyzed. The result showed that "Yongyou2640" and "Xinrongyouhua" showed moderate growth period in the main cropping season, regular ears in the ratooning season, high yield and high quality in two cropping seasons and could be recommended as main machine-harvesting ratoon rice variety in Pucheng County. "Jiayou99" showed relatively long growth period in the main season, low yield but high regenerative capacity and high yield in the ratooning season and could be a machine-harvesting ratoon rice varieties in southern and central Pucheng County.