Abstract: In this paper, effects of Red-leaf cattle manure fertilizer on growth and development of tobacco were studied. The results showed that Red-leaf cattle manure fertilizer could enhance growth and development of tobacco plants and improve yield and quality and maturity of tobacco leaf. Production value was 3256.59 yuan per 667 m² and higher than the treatments applying common fertilizer and rape seed cake fertilizer by 19.67% and 3.42%, respectively.