中华猕猴桃茎段组培快繁系列技术研究

Tissue culture and rapid propagation technique for Actinidia chinensis

摘要: 以中华弥猴桃带腋芽茎段为外植体，研究不同处理方法对外植体灭菌效果的影响，不同激素组合培养基对外植体初代培养和组培苗生根的影响。试验结果表明，最佳灭菌处理：先用75%酒精灭菌30 s，再用0.1%升汞进行表面灭菌8 min，外植体成活率88%；最佳初代培养基：MS+6-BA 1.5 mg/L+NAA 0.2 mg/L，出芽率92%；最佳生根培养基：1/2 MS+IBA 0.15 mg/L+NAA 0.3 mg/L，生根率85.6%。

Abstract: Effects of different treatments on explant sterilization, different hormone formulation on initial culture of explant and rooting of tissue culture seedling, were studied, using Actinidia chinensis stem with axillary bud as explant. The results showed that the best sterilizaiton treatment was 75% alcohol for 30 s firstly, and then 0.1% Mercury(II) chloride for 8 min, and the survival rate of explant reached 88%. The optimum initial culture medium was MS+6-BA 1.5 mg/L+NAA 0.2 mg/L, the budding rate of which was 92%. The optimum rooting culture medium was 1/2 MS+IBA 0.15 mg/L+NAA 0.3 mg/L, with 85.6% of the rooting rate.