Abstract: A test on reduced application of chemical fertilizer and combining application of bio-organic fertilizer of edible fungi residue was carried out.The results showed that the treatment of reducing the amount of chemical fertilizer by 20%-25% and combining application of bio-organic fertilizer of edible fungi residue could improve the disease resistance of tobacco plants,reduce the incidence of rhizome disease,reduce the nicotine content of tobacco leaves,the smoke of tobacco leaves is more transparent,smooth,and the aftertaste is comfortable.