Abstract: A kind of natural selenium-rich containing grape was used to produce selenium-enriched wine, the results showed that after 7-month fermentation of the selenium-rich containing grapes, the content of selenium in grape wine was about 27 mu g/kg, and about 13% selenium in the grapes were migrated to wine. Three different fermentation methods had some influence on selenium content in fermentation process, but the final content of selenium in wine was basically consistent.