16种液体处理剂对铁皮石斛组培苗促生作用研究

    Effect of 16 Liquid Treatment Agents on Stimulating Growth of Tissue Culture Plantlets of Dendrobium officinale

      摘要
    • 摘要: 选用玉蜀黍赤霉、枯草芽孢杆菌、石斛小菇3种细菌和真菌菌株以及与水杨酸、硝酸镧、色氨酸、赤霉素结合配制16种液体处理剂，并以灌根方式处理，接种大棚铁皮石斛组培苗。结果表明：4 g·L-1石斛小菇、5 mg·L-1赤霉素、4 g·L-1石斛小菇+5 mg·L-1硝酸镧、4 g·L-1石斛小菇+5 mg·L-1赤霉素+1 mmol·L-1水杨酸处理的铁皮石斛组培苗株高显著高于对照组（无菌水处理）；4 g·L-1石斛小菇、5 mg·L-1赤霉素、1 mmol·L-1水杨酸、4 g·L-1石斛小菇+5 mg·L-1硝酸镧、4 g·L-1石斛小菇+5 mg·L-1赤霉素+1 mmol·L-1水杨酸茎粗显著高于对照组。说明接种4 g·L-1石斛小菇、5 mg·L-1赤霉素、4 g·L-1石斛小菇+5 mg·L-1硝酸镧、4 g·L-1石斛小菇+5 mg·L-1赤霉素+1 mmol·L-1水杨酸液体处理剂处理能有效提高幼苗的株高、茎粗，液体处理剂有良好的促生效果。

       

      Abstract: Sixteen liquid treatment agents were formulated with the combination of three bacteria or fungal, Gibberella zeae, Bacillus subtilis and Mycena dendrobii, and salicylic acid, lanthanum nitrate, tryptophan and gibberellin, and treated tissue culture seedling of Dendrobium officinale in greenhouse by root irrigation. The results showed that the treatments of 4 g·L-1 Mycena dendrobii, 5 mg·L-1 gibberellin, 4 g·L-1 Mycena dendrobii+5 mg·L-1 lanthanum nitrate, 4 g·L-1 Mycena dendrobii+5 mg·L-1 gibberellic acid+1 mmol·L-1 salicylic acid could significantly increase the plant height of Dendrobium officinale compared to the control (sterile water treatment); The stem diameter of 4 g·L-1 Mycena dendrobii, 5 mg·L-1 gibberellin, 1 mmol·L-1 salicylic acid, 4 g·L-1 Mycena dendrobii+5 mg·L-1 lanthanum nitrate, 4 g·L-1 Mycena dendrobii+5 mg·L-1 lanthanum nitrate treatments were significantly higher than that of the control group. It was indicated that the treatments of 4 g·L-1 Mycena dendrobii, 5 mg·L-1 gibberellin, 4 g·L-1 Mycena dendrobii+5 mg·L-1 lanthanum nitrate, 4 g·L-1 Mycena dendrobii+5 mg·L-1 gibberellic acid+1 mmol·L-1 salicylic acid could increase the plant height and stem diameter effectively, the liquid treatment agents of which had good growth-stimulating effects on tissue culture plantlets of Dendrobium officinale in greenhouse.

       

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