Abstract: For promoting the development of mushroom stalk industry, the effects of temperature, concentration, salt ion and pH on the solubility of polysaccharides were investigated with the polysaccharide of mushroom stalk as the material. The results showed that the polysaccharide of mushroom stalk was greatly influenced by temperature and concentration, and the suitable dissolution temperature was 60℃. And which was less affected by the salt ions and dissolved in the mono-valence and bi-valence inorganic salt solution. In addition, the solubility of the polysaccharide under the alkaline condition was higher than the environment of acid and neutral, and the solubility in the environment of acid and neutral changed little.