Abstract: In order to enhance breeding of new sugarcane cultivar, new-planting and ratooning test of new sugarcane line ‘Yuetang 00/237’ were conducted. The results showed that the comprehensive evaluation score of ‘Yuetang 00/237’ in Xinhe Sugarcane Production Area of Chongzuo City surpass that of control variety ‘Xintaitang No.22’. And it showed stable comprehensive performance and had characteristics including good ratoon ability, high adverse-resistance, stable yield and high sugar content. Its yield, sucrose content and sugar content when new planting were 104.5 t·hm^-2, 15.71% and 16.42 t·hm^-2 while its yield, sucrose content and sugar content when ratooning were 98.72 t·hm^-2, 16.36% and 16.15 t·hm^-2. ‘Yuetang 00/237’ had good comprehensive performance in Chongzuo Sugarcane Production Area with high popularization and application value.