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固相萃取高效液相色谱荧光法测定炒制坚果中苯并(a)芘残留量

陶华平

陶华平. 固相萃取高效液相色谱荧光法测定炒制坚果中苯并(a)芘残留量[J]. 福建农业科技, 2018, 49(6): 36-39. DOI: 10.13651/j.cnki.fjnykj.2018.06.011
引用本文: 陶华平. 固相萃取高效液相色谱荧光法测定炒制坚果中苯并(a)芘残留量[J]. 福建农业科技, 2018, 49(6): 36-39. DOI: 10.13651/j.cnki.fjnykj.2018.06.011
TAO Hua-ping. Determination of Benzoapyrene Residue in Stir-fried Nuts by Solid Phase Extraction and High Performance Liquid Chromatography Fluorescence Spectrometry[J]. Fujian Agricultural Science and Technology, 2018, 49(6): 36-39. DOI: 10.13651/j.cnki.fjnykj.2018.06.011
Citation: TAO Hua-ping. Determination of Benzoapyrene Residue in Stir-fried Nuts by Solid Phase Extraction and High Performance Liquid Chromatography Fluorescence Spectrometry[J]. Fujian Agricultural Science and Technology, 2018, 49(6): 36-39. DOI: 10.13651/j.cnki.fjnykj.2018.06.011

固相萃取高效液相色谱荧光法测定炒制坚果中苯并(a)芘残留量

详细信息
    作者简介:

    陶华平,男,1985年生,质量工程师。

Determination of Benzoapyrene Residue in Stir-fried Nuts by Solid Phase Extraction and High Performance Liquid Chromatography Fluorescence Spectrometry

  • 摘要: 建立高效液相色谱-荧光检测器测定炒制坚果中苯并(a)芘残留量的方法,样品采用正已烷∶丙酮(V∶V=3∶1)混合溶剂萃取后,经中性氧化铝柱净化后用配备荧光检测器高效液相色谱仪进行测定。结果表明,苯并(a)芘在0.50~10.00 ng·mL-1范围内线性良好,线性方程y=119 649x+1 831.08,相关系数为0.999 98。在3个不同浓度水平(1.00、5.00、10.00 ng·mL-1)添加标样,回收率为80.4%~87.2%,相对标准偏差为3.5%~4.2%。结果表明,该方法灵敏、简便、精确度高,可满足炒制坚果中苯并(a)芘残留量检测。
    Abstract: To establish a method for determing benzoapyrene residue in stir-fried nuts by HPLC-fluorescence detector, the sample was extracted with n-hexane and acetone (V:V=3:1) mixed solvent, purified by neutral alumina column and determined by HPLC equipped with fluorescence detector. The results showed that the linearity of benzoapyrene was good in the range of 0.50-10.00 ng·mL-1 with the linear equation y=119649 x+1831.08 and the correlation coefficient 0.99998. The average recovery was 80.4%-87.2% and the relative standard deviation was 3.5%-4.2% when standard samples were added at 3 different concentration levels (1.00, 5.00, 10.00 ng·mL-1). The results showed that the method was sensitive, simple and accurate, and could meet the requirement for the determination of benzoapyrene residues in stir-fried nuts.
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出版历程
  • 收稿日期:  2018-05-27
  • 刊出日期:  2018-06-27

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