水稻OsTZF3基因敲除和过量表达载体的构建与遗传转化

    Construction and Genetic Transformation of OsTZF3 Gene Knockout and Overexpression Vectors in Rice

      摘要
    • 摘要: 为进一步研究水稻OsTZF3基因的生物学功能，利用日本晴基因组的OsTZF3基因序列，构建了OsTZF3的CRISP/Cas9敲除表达载体pKTZF3和过量表达载体pCXUN-TZF3；并通过农杆菌介导法导入粳稻日本晴胚性愈伤组织中，分别获得了pKTZF3和pCXUN-TZF3转化再生植株55个和42个克隆；进一步通过PCR法鉴定了35个克隆转pKTZF3植株和10个克隆转pCXUN-TZF3植株。

       

      Abstract: In order to further study the biological function of OsTZF3 gene in rice, the CRISP/Cas9 knockout expression vector pKTZF3 and overexpression vector pCXUN-TZF3 of OsTZF3 were constructed by using the sequence of OsTZF3 gene in Nipponbare. 55 transformants of pKTZF3 and 42 clones of pCXUN-TZF3 were obtained through Agrobacterium-mediated transformation. Among them, 35 clones of pKTZF3 and 10 clones of pCXUN-TZF3 were further identified by PCR detection.

       

      • HTML全文
      • 参考文献(9)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回