Abstract: Four rice cultivars with different indica and japonica components were used as experimental materials, and four induction media were designed with different basic medias (GM₈, N₆) and hormones (single hormone, complex hormones) as variables to study the suitable conditions for anther culture of rice. The results showed that GM₈ medium had better effect for anther culture of indica rice and indica-cline type, and N₆ medium had better effect for anther culture of Japonica rice. The number and developmental condition of callus from medium treated with complex hormones were better than that treated with single hormone, which indicated that complex hormones treatment had higher callus rate and better anther culture effect.