花叶金线莲离体保存技术研究

Study on the Technique of in Vitro Conservation for Mosaic Anoectochilus Formosanus

摘要: 采用缓慢生长保存法对花叶金线莲进行离体保存试验。结果表明：在培养温度（24±3）℃，光照强度前3个月1 500~2 000 lx、3个月后500~1 000 lx，光照时间10 h·d^-1的培养条件下，花叶金线莲单芽在改良MS+蔗糖20 g·L^-1+甘露醇20 g·L^-1培养基中保存12个月，存活率达85.0%，保存后能恢复正常生长；缓慢生长保存法可为金线莲种质保存提供新的方法与思路。

Abstract: The method of slow growth conservation was used to conduct the experiment of in vitro conservation of mosaic anoectochilus formosanus. The results showed that:In the culture conditions of the temperature being 24±3℃, the illumination intensity being 1 500-2 000 lx for the first 3 months and 500-1 000 lx after 3 months, and the illumination time being 10 h·d^-1, the survival rate of the single bud of mosaic anoectochilus formosanus reached 85.0% after 12 months conservation in the modified MS+sucrose 20 g·L^-1+ mannitol 20 g·L^-1 medium. And it could restore normal growth after the conservation, which provided a new method and idea for the germplasm conservation of anoectochilus formosanus.