Abstract: In order to further research the effects of the culture conditions of liquid seed production on red yeast rice with high color value, improve the quantity and quality of monascus mycelium in liquid seed solution, shorten the fermentation period and promote the large-scale production of high-quality red yeast rice, the main culture conditions such as culture cycle, culture temperature, inoculum size, initial pH value of culture medium and loaded liquid were optimized. The results showed that the optimal culture conditions for liquid seed production of monascus were the initial pH of liquid seed production medium of 5.5, inoculum size of 6%, culture temperature at 35℃ and culture cycle of 24 h. After optimizing the liquid seed production process of monascus, the color value of red yeast rice was up to 6 000-6 200 u·g^-1, the fermentation period was 10-12 d, and the production yield was up to 35%-38%.