Abstract:
In order to develop and utilize the byproducts of
Sparassis crispa and increase the added value of hydrangea, the effects of extraction temperature, extraction time and ratio of feed to water on the polysaccharide yield of byproducts of
Sparassis crispa were investigated. On this basis, the model of response surface analysiswas established, and by linear regression fitting, the regression model was obtained:polysaccharide yield (%)=7.19+0.26A-0.35B-0.84C-0.088AB-0.57AC+0.54BC-0.35A
2-3.38B
2-0.42C
2,
P<0.0001, reaching a very significant level. The lack of fit (
P=0.5101) was not significant, indicating that the model was valid. Then, the model was optimized by using Design-Expert8.0 software, with the highest polysaccharide yield as the optimization target, and the optimal technological conditions for extracting polysaccharides from the byproducts of
Sparassis crispa were as follows:extraction time was 3.83 h, extraction temperature was 78.75℃, and the solid-liquid ratio 1:30, thus the polysaccharide yield could be up to 7.89%. According to the actual situation, the technological parameters were revised as follows:extraction time was 3.83 h, extraction temperature was 79.00℃, and the solid-liquid ratio was 1:30. The polysaccharides were extracted from the byproducts of
Sparassis crispa under the optimal conditions, and the actual polysaccharide yield was 8.01%.