全细胞催化生产γ-氨基丁酸转化液脱色工艺研究

    Decoloring Technology of the Conversion Solution for Producing γ-aminobutyric Acid by Whole-cell Catalysis

      摘要
    • 摘要: 针对本实验室1株高产γ-氨基丁酸（γ-aminobutyric acid，简称GABA）的大肠杆菌，以麸酸为底物利用全细胞催化的方法生产GABA，研究了生产GABA的转化液脱色工艺。试验采用活性炭结合树脂层析的脱色方法，首先对14种脱色树脂的脱色能力进行筛选，结果表明FPA53阴离子交换树脂的脱色效果显著优于其他树脂，通过单因素试验对树脂的脱色温度、pH进行优化，得到FPA53树脂的理想脱色条件与转化液初始条件相近，为温度40℃、pH6。在该条件下，转化液的脱色率达到了85.1%，GABA回收率达到98%以上，谷氨酸去除率达到23%以上。对6种活性炭进行筛选，结果表明2号竹清活性炭的脱色效果显著优于其他活性炭，通过单因素试验对活性炭的添加比例进行优化，得到1%的活性炭添加比例为试验理想比例，在此条件下，转化液的脱色率达到92.8%以上，GABA回收率达到99%，而液体损失率只有2%。最后，将筛选得到的活性炭与脱色树脂结合使用，结果显示，结合使用后的转化液脱色率可以达到99.5%以上，GABA回收率达到98%以上，结晶颗粒洁白无杂色。该研究结果为GABA精制及其大规模工业生产提供基础。

       

      Abstract: Aiming at a strain of colibacillus with high-yielding γ-aminobutyric acid (GABA) in our laboratory, the whole-cell catalysis method was used to produce GABA with glutamic acid as the substrate, and the decolorization process of the conversion solution for the production of GABA was studied. In the experiment, the decolorization method of active carbon combined with resin chromatography was used. Firstly, the decolorization ability of 14 kinds of decolorizing resins was screened. The results showed that the decolorization effect of FPA53 anion-exchange resin was significantly better than that of other resins. Through the single factor test, the decolorization temperature and pH of the resin were optimized, and the ideal decolorization condition of FPA53 resin was similar to the initial condition of the conversion solution, with a temperature of 40℃ and a pH of 6. Under these conditions, the decolorization rate of the conversion liquid reached over 85.1%, the recovery rate of GABA reached over 98%, and the removal rate of glutamic acid reached over 23%. The six kinds of activated carbons were screened, and the results showed that the decolorization effect of No.2 Zhuqing activated carbon was significantly better than that of other activated carbons. The appending proportion of activated carbon was optimized through the single factor experiment, and the appending proportion of 1% activated carbon was the ideal proportion of the test. Under these conditions, the decolorization rate of the conversion solution reached over 92.8%, the recovery rate of GABA reached 99%, and the liquid loss ratio was only 2%. Finally, the selected activated carbon was combined with the decolorizing resin, and the results showed that the decolorization rate of the conversion solution after the combined use could reach over 99.5%, the recovery rate of GABA could reach over 98%, and the crystalline particles were white and no variedness. The results of this study provided a basis for GABA refining and its large-scale industrial production.

       

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