Abstract: The contents of 10 elements (Mg, Ca, Cu, Zn, Mn, Fe, Cd, Cr, Pb, Hg) in Laminaria japonica samples were determined simultaneously by using the inductively coupled plasma source mass spectrometer after the treatment of microwave digestion in nitric acid solution, and the linear relationship, detection limit, precision and adding standard recovery of the method were also investigated. The results showed that the linear relationship of 10 elements in Laminaria japonica was good, the correlation coefficient r was all greater than 0.999 2, the adding standard recovery was 88.0%-102.1%, and the relative standard deviation (RSD) was 4.0%-6.8%. The national standard reference materials were selected to verify the accuracy of the method, finding that the determination results of 10 elements were all within the allowable range of national standard values. The method was simple to operate, and fast to analyze, with good recovery rate, high precision, and accurate & reliable determination results, which could satisfy the simultaneous determination and analysis of various elements in Laminaria japonica, and could provide reference for the quality control and safety evaluation of Laminaria japonica.