Optimization on Purification Agent Combination of 5 kinds of Pesticide Residues in Strawberry Measured by QuEChERS-Gas Chromatography
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摘要: 为建立草莓中5种农药残留QuEChERS-气相色谱检测方法。用乙腈对草莓中的农药进行提取，选取适量的乙二胺-N-丙基硅烷（PSA）、无水硫酸镁、石墨炭黑（GCB）进行分散固相萃取，净化液过滤膜后上GC-ECD检测，利用SPSS statistics version 19软件进行正交试验设计及方差分析。结果表明：最优的净化组合为A3B3C2，即乙二胺-N-丙基硅烷（PSA）125 mg、石墨炭黑（GCB）50 mg、无水硫酸镁250 mg，腐霉利、咪鲜胺、百菌清、毒死蜱、氟氯氰菊酯回收率分别为96.5%、94.8%、97.6%、99.1%、97.9%。通过优化QuECHERS-气相色谱测定的净化剂组合，该检测方法适用于草莓中腐霉利等5种农药残留的检测。Abstract: In order to establish a method for the determination of 5 kinds of pesticide residues in strawberries by QuEChERS-gas chromatography, the acetonitrile was used to extract the pesticide from strawberries, and the appropriate amount of ethylenediamine-n-propylsilane (PSA), anhydrous magnesium sulfate, and graphitized carbon black (GCB) were selected for the dispersive solid-phase extraction. GC-ECD detection was conducted on the filtering membrane of the purification solution. The orthogonal test design and variance analysis were conducted by using the software of SPSS statistics version 19. The results showed that the optimal purification combination was A3B3C2, that was, ethylamine-n-propylsilane (PSA) 125 mg, graphite carbon black (GCB) 50 mg, and anhydrous magnesium sulfate 250 mg, and the recoveries of procymidone, prochloraz, chlorothalonil, chlorpyrifos and cyfluthrin were 96.5%, 94.8%, 97.6%, 99.1% and 97.9%, respectively. The method was suitable for the detection of 5 kinds of pesticide residues such as procymidone in strawberries by optimizing the combination of purification agent measured by QuEChERS-gas chromatography.
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Keywords:
- Strawberry /
- Pesticide residues /
- QuEChERS /
- Orthogonal test /
- Gas chromatography
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