球等鞭金藻中岩藻黄素的提取及其纯化工艺研究

陈建楠; 陈由强; 薛婷

doi:10.13651/j.cnki.fjnykj.2020.04.005

球等鞭金藻中岩藻黄素的提取及其纯化工艺研究

陈建楠^1,2,
陈由强^1,2,
薛婷^1,2, ,

1. 福建师范大学生命科学学院, 福建福州 350117;
2. 福建师范大学南方海洋研究院, 福建福州 350117

基金项目:

国家糖料产业技术体系（CARS-170501）；2020年福建省教育厅中青年教师科研项目（JAT190091）。

详细信息

作者简介:
陈建楠,男,1991年生,硕士,主要从事海洋微藻培育研究。

通讯作者:
薛婷,女,1988年生,博士,主要从事海洋微藻培育和基因组生物信息学分析(E-mial:xueting7872126@163.com)。

中图分类号: S963
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出版历程
- 收稿日期: 2020-03-24
- 刊出日期: 2020-04-27

Study on the Extraction and Purification Process of Fucoxanthin in Isochrysis Galbana

1. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China;
2. Southern Institute of Oceanography, Fujian Normal University, Fuzhou, Fujian 350117, China

摘要

摘要: 以球等鞭金藻藻粉为原料，研究了球等鞭金藻中的岩藻黄素有机溶剂提取条件工艺优化、硅胶层析柱分离纯化和超高效液相色谱-串联质谱联用仪定性分析。主要通过单因素试验法和正交优化法确定了提取条件的工艺优化参数，硅胶层析柱分离纯化岩藻黄素，并通过超高效液相色谱（UPLC）定量检测和LCQ Fleet离子阱液质联用仪定性分析岩藻黄素。试验结果表明，最佳提取工艺为提取温度35℃、料液比1：30（g/mL）、提取时间15 min、提取次数2次，岩藻黄素的提取率达到12.03 mg·g^-1。硅胶层析柱分离纯化后最佳回收率为86.35%，纯度为29.78%，LCQ Fleet离子阱液质联用仪定性分析质量分数为659.090 2。本研究结果为工业化快速提取球等鞭金藻中的岩藻黄素活性物质提供了理论基础。
- 球等鞭金藻 /
- 岩藻黄素 /
- 提取工艺 /
- 纯化
Abstract: By taking Isochrysis galbana powder as the raw material, the process optimization of extraction conditions with the organic solvent of fucoxanthin in Isochrysis galbana, the separation and purification by silica column chromatography, and the qualitative analysis by UPLC-GC-MS were studied in this paper. The process optimization parameters of extraction conditions were determined mainly by the methods of single factor test and orthogonal optimization, and then the fucoxanthin was separated and purified by silica column chromatography. Last, the fucoxanthin was quantitatively detected by UPLC and qualitatively analyzed by LCQ Fleet. The results showed that the optimum extraction process was as follows:the extraction temperature was 35℃, the solid-liquid ratio was 1:30(g/mL), the extraction time was 15 min, and the extraction times were twice. Under these conditions, the extraction rate of fucoxanthin reached 12.03 mg·g^-1, the optimal recovery rate was 86.35% and the purity was 29.78% after the separation and purification by silica column chromatography, and the mass fraction qualitatively analyzed by LCQ Fleet was 659.090 2. The results of this study provided a theoretical basis for the rapid industrial extraction of the active substance of fucoxanthin from Isochrysis galbana.
- Isochrysis galbana /
- Fucoxanthin /
- Extraction process /
- Purification

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