Study on the Extraction and Purification Process of Fucoxanthin in Isochrysis Galbana
-
摘要: 以球等鞭金藻藻粉为原料,研究了球等鞭金藻中的岩藻黄素有机溶剂提取条件工艺优化、硅胶层析柱分离纯化和超高效液相色谱-串联质谱联用仪定性分析。主要通过单因素试验法和正交优化法确定了提取条件的工艺优化参数,硅胶层析柱分离纯化岩藻黄素,并通过超高效液相色谱(UPLC)定量检测和LCQ Fleet离子阱液质联用仪定性分析岩藻黄素。试验结果表明,最佳提取工艺为提取温度35℃、料液比1:30(g/mL)、提取时间15 min、提取次数2次,岩藻黄素的提取率达到12.03 mg·g-1。硅胶层析柱分离纯化后最佳回收率为86.35%,纯度为29.78%,LCQ Fleet离子阱液质联用仪定性分析质量分数为659.090 2。本研究结果为工业化快速提取球等鞭金藻中的岩藻黄素活性物质提供了理论基础。Abstract: By taking Isochrysis galbana powder as the raw material, the process optimization of extraction conditions with the organic solvent of fucoxanthin in Isochrysis galbana, the separation and purification by silica column chromatography, and the qualitative analysis by UPLC-GC-MS were studied in this paper. The process optimization parameters of extraction conditions were determined mainly by the methods of single factor test and orthogonal optimization, and then the fucoxanthin was separated and purified by silica column chromatography. Last, the fucoxanthin was quantitatively detected by UPLC and qualitatively analyzed by LCQ Fleet. The results showed that the optimum extraction process was as follows:the extraction temperature was 35℃, the solid-liquid ratio was 1:30(g/mL), the extraction time was 15 min, and the extraction times were twice. Under these conditions, the extraction rate of fucoxanthin reached 12.03 mg·g-1, the optimal recovery rate was 86.35% and the purity was 29.78% after the separation and purification by silica column chromatography, and the mass fraction qualitatively analyzed by LCQ Fleet was 659.090 2. The results of this study provided a theoretical basis for the rapid industrial extraction of the active substance of fucoxanthin from Isochrysis galbana.
-
Keywords:
- Isochrysis galbana /
- Fucoxanthin /
- Extraction process /
- Purification
-
-
[1] 臧正蓉.富含岩藻黄素的微藻藻种的筛选及其中试培养[D].北京:中国科学院研究生院(海洋研究所),2014. [2] 刘春凤,吴雪,高悦勉.两种等鞭金藻外部形态与超微结构的观察比较[J].大连水产学院学报,2008(4):273-277. [3] 王帅.富油微藻筛选及球等鞭金藻(Isochrysis galbana)脂肪酸去饱和酶基因的克隆与功能研究[D].青岛:中国海洋大学,2015. [4] 汪曙晖,薛长湖.岩藻黄素的结构、性质和功能[J].食品工业科技,2010,31(6):408-410,407. [5] 张文源,高保燕,雷学青,等.岩藻黄素的理化与生物学特性、制备技术及其生理活性研究进展[J].中国海洋药物,2015,34(3):81-95. [6] 周卫松.裙带菜中岩藻黄质、岩藻多糖的综合提取纯化研究[D].杭州:浙江大学,2014. [7] ZHANG Y P,FANG H,XIE Q L,et al.Comparative Evaluation of the Radical-Scavenging Activities of Fucoxanthin and Its Stereoisomers[J].Molecules,2014,19:2100-2113.
[8] 汪曙晖.海藻中岩藻黄素的分离鉴定及抗肿瘤活性研究[D].青岛:中国海洋大学,2010. [9] XIA S,WANG K,WAN L,et al.Production, Characterization, and Antioxidant Activity of Fucoxanthin from the Marine Diatom Odontella aurita[J].Marine Drugs,2013,11(7):2667.
[10] NISHINO H.Cancer prevention by carotenoids[J].Archives of Biochemistry & Biophysics,1998,402(1-2):159-163.
[11] SUGAWARA T,BASKARAN V W,NAGAO A.Brown algae fucoxanthin is hydrolyzed to fucoxanthinol during absorption by Caco-2 human intestinal cells and mice[J].Journal of Nutrition,2002,132(5):946-951.
[12] HOSOKAWA M,KUDO M,MAEDA H,et al.Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARγ ligand,troglitazone,on colon cancer cells[J].Biochimica Et Biophysica Acta General Subjects,2004,1675(3):113-119.
[13] KIM J C.Solvent Extraction of Fucoxanthin from Phaeodactylum tricornutum[J].Separation Science & Technology,2014,49(3):410-415.
[14] WANG W J,WANG G C,ZHANG M,et al.Isolation of Fucoxanthin from the Rhizoid of Laminaria japonica Aresch[J].Journal of Integrative,2005,47(8):1009-1015.
[15] 尹尚军,徐涛,刘丽平,等.羊栖菜岩藻黄质的提取工艺研究[J].食品工业科技,2011(4):272-275. [16] ROH M K,CHUN U B S.Extraction of fucoxanthin and polyphenol from Undaria pinnatifida using supercritical carbon dioxide with co-solvent[J].Biotechnology & Bioprocess Engineering,2008,13(6):724-729.
[17] XIAO X,SI X,YUAN Z,et al.Isolation of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography[J].Journal of Separation Science,2012,35(17):2313-2317.
[18] 陈文佳.海带中岩藻黄素提取工艺优化及性质研究[D].济南:山东轻工业学院,2012. [19] MARCHAL L,LEGRAND J,FOUCAULT A.Centrifugal partition chromatography:A survey of its history,and our recent advances in the field[J].Chemical Record,2003,3(3):133-143.
[20] PIOVAN A,FILIPPINI R,DE P M,et al.TLC densitometric method for the preliminary evaluation of fucoxanthin-based products[J].Natural Product Research,2014,28(14):1111-1115.
[21] RAJAURIA G,ABU-GHANNAM N.Isolation and Partial Characterization of Bioactive Fucoxanthin from Himanthalia elongata Brown Seaweed:A TLC-Based Approach[J].International Journal of Analytical Chemistry,2013,2013(1):4518-4519.
[22] SUDHAKAR M P.Extraction, purification and study on antioxidant properties of fucoxanthin from brown seaweeds[J].Journal of Chemical & Pharmaceutical Research,2013,5(7):169-175.
[23] HOSOKAWA M,WANEZAKI S,MIYAUCHI K,et al.Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60[J].Food Science & Technology Research,2006,5(3):243-246.
-
期刊类型引用(6)
1. 朱军旺,叶月美,周成旭,张金荣. 三角褐指藻中岩藻黄素的分离纯化工艺研究. 中国海洋药物. 2024(04): 19-27 . 百度学术
2. 林港华,李欢,韩玉莹,薛婷,李龙玉,代容春,林荣华,陈建楠,陈由强,余雪兵. 不同光质对球等鞭金藻岩藻黄素及脂肪酸含量的影响. 福建农业科技. 2022(10): 25-31 . 本站查看
3. 李敏兰,弓泽华,盛滟,凌俊红. 岩藻黄素提取与分析方法研究进展. 食品研究与开发. 2021(03): 202-206 . 百度学术
4. 金旭东,王俊淇,曹朝清,王碧,丁浩淼,汪财生,钱国英. 循环超声提取羊栖菜中岩藻黄质的工艺研究. 食品工业科技. 2021(17): 170-178 . 百度学术
5. 许燕茹,林岗. 恩诺沙星对球等鞭金藻生长及脂肪酸的影响. 福建农业科技. 2021(09): 8-14 . 本站查看
6. 花汝凤,周淑华. 高效液相色谱法测定海带浓缩制品中岩藻黄素的含量. 食品安全质量检测学报. 2020(19): 7114-7117 . 百度学术
其他类型引用(4)
计量
- 文章访问数: 395
- HTML全文浏览量: 0
- PDF下载量: 34
- 被引次数: 10