Abstract: In order to identify the high yield, stability and adaptability of the new strain X1 of Hypsizigus marmoreus, and provide reference for the subsequent promotion and application of the strain, the main strain HX-1 of Hypsizigus marmoreus cultivated in Gutian County was selected as the control group, and the production performance of the new strain X1 of Hypsizigus marmoreus was evaluated through the cultivation cycle, the yield of fruiting body, and agronomic traits, etc. The results showed that the fruiting body of the new strain X1 of Hypsizigus marmoreus was cespitose, while the pileus was white and the stipe was white and hard. Compared with the strain HX-1, the cultivation cycle of the new strain was shortened by more than 10 days, with synchronous production and fruiting regularly. The average yield per unit of fresh mushroom was 676.52 g per bag, which was 5.86% higher than that of the strain HX-1. In conclusion, the new strain X1 of Hypsizigus marmoreus had good value for production and application.