Abstract: In order to select the suitable cultivation substrates for the growth of Lengfentiaowen, the variety of Dendrobium nobile, the effects of different substrate formulations on the tillering rate and new bud height of Lengfentiaowen by using the fermented pine bark and perlite with different proportions as the culture substrates and taking the pure water moss culture as the control group. The results showed that in May, June and August, the tillering rate of Lengfentiaowen was higher under the culture substrate with the ratio of fermented pine bark to perlite being 10:2, which was not significantly different from that with the ratio of fermented pine bark to perlite being 10:1, pure fermented pine bark and pure water moss. In May and June, the height of new buds of Lengfentiaowen under the culture substrate with the ratio of fermented pine bark to perlite being 10:3 was significantly higher than that of the control group. But there was no significant difference between the other treatments and the control group in each month. Lengfentiaowen had the highest height of new buds while maintaining a higher tillering rate under the culture substrate with the ratio of fermented pine bark to perlite being 10:3. Therefore, the formulation with the ratio of fermented pine bark to perlite being 10:3 was the most beneficial to the tillering and bud growth of Lengfentiaowen.