超微粉碎纤维素酶法改性竹笋膳食纤维的工艺评价

    Process Evaluation of the Modification of Dietary Fiber in Bamboo Shoots by Ultra-fine Pulverization-Cellulase Method

    • 摘要: 以竹笋为原料,评价超微粉碎-纤维素酶法对膳食纤维(DF)改性的影响。通过检测竹笋可溶性膳食纤维(SDF)的得率、持水力以及膨胀力,分析超微粉碎法粉碎时间(0~25 s)、纤维素酶浓度(0.1%~0.5%)、酶解时间(30~150 s)、酶解温度(30~55℃)、pH值(4.5~5.5)对竹笋膳食纤维改性的影响;通过Box-Behnken中心组合试验设计方法,将酶浓度、酶解温度、酶解时间以及样品pH值因素作为自变量,并以样本SDF得率作为响应值,构建四因素三水平Box-Behnken模型,分析竹笋DF改性工艺。结果表明:经超微粉碎竹笋DF的性质与未改性相比显著更优(P<0.05),粉碎时间0~10 s内,随超微粉碎时间的延长,竹笋SDF得率、持水力与膨胀性升高。经超微粉碎处理10 s 后SDF得率(8.60±0.142)%、持水力(5.11±0.050) g·g-1、膨胀性(11.06±0.067)mL·g-1。随超微粉碎的时间进一步延长,竹笋SDF得率、持水力与膨胀性开始下降;在纤维素酶0.1%~0.3%浓度范围内,随着纤维素酶的浓度升高,竹笋SDF得率、持水力及膨胀力均逐渐增大,当纤维素酶的浓度>0.3%,随着纤维素酶的浓度升高,竹笋SDF得率、持水力及膨胀力均逐渐下降;酶解时间60 min,竹笋SDF得率、持水力及膨胀力最佳;当pH达到4.5,竹笋SDF得率、持水力及膨胀力最佳;对竹笋SDF得率影响中最显著因素为纤维素酶浓度(F=28.76),其次为pH(F=18.05)、酶解时间(F=11.43)、酶解温度(F=10.93);Design-Expert回归模型分析显示,酶浓度0.32%、酶解时间85.03 min、酶解温度为50℃,pH值4.81,竹笋SDF得率预测值达到15.89%。

       

      Abstract: By taking the bamboo shoots as raw materials, the effect of ultra-fine pulverization-cellulase method on the modification of dietary fiber (DF) was evaluated. By detecting the yield, water-holding power and expansive force of soluble dietary fiber (SDF) in bamboo shoots, the effects of ultra-fine pulverization time (0-25 s), cellulase concentration (0.1%-0.5%), enzymolysis time (30-150 s), enzymolysis temperature (30-55℃) and pH value (4.5-5.5) on the modification of dietary fiber in bamboo shoots were analyzed. By taking the enzymolysis concentration, enzymolysis temperature, enzymolysis time and pH value of samples as the independent variables, and the yield of SDF in the samples as the response value, the Box-Behnken central composite design method was used to construct a Box-Behnken model with 4 factors and 3 levels, thus to analyze the DF modification technology of bamboo shoots. The results showed that the properties of DF in bamboo shoots after ultra-fine pulverization were significantly better than those without modification (P<0.05). The yield, water-holding power and expansibility of SDF in bamboo shoots increased with the extension of ultra-fine pulverization time within 0-10 s. The yield of SDF was (8.60±0.142) %, the water-holding power was (5.11±0.050) g·g-1, and the expansibility was (11.06±0.067) mL·g-1 after ultra-fine pulverization for 10 s. The yield, water-holding power and expansibility of SDF in bamboo shoots began to decrease with the further extension of ultra-fine pulverization time. In the range of cellulase concentration from 0.1% to 0.3%, the yield, water-holding power and expansive force increased with the increase of cellulase concentration. When the cellulase concentration was greater than 0.3%, the yield, water-holding power and expansive force of SDF in bamboo shoots decreased with the increase of the cellulase concentration. The best yield, water-holding power and expansive force of SDF in bamboo shoots were obtained after enzymatic hydrolysis for 60 min. When the pH reached 4.5, the yield, water-holding power and expansive force of SDF in bamboo shoots were the best. The most significant factor affecting the yield of SDF in bamboo shoots was the cellulase concentration (F=28.76), followed by the pH (F=18.05), enzymolysis time (F=11.43) and enzymolysis temperature (F=10.93). The Design Expert regression model analysis showed that the predicted yield of SDF in bamboo shoots reached 15.89% with the cellulase concentration being 0.32%, enzymolysis time being 85.03 min, enzymolysis temperature being 50℃, and pH value being 4.81.

       

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