Abstract: In order to screen the good strains of Pleurotus geesteranus which were suitable for the cultivation of Wokou fungus sack in Luoyuan County of Fujian Province, six strains of Pleurotus geesteranus were introduced for the comparative experiment, so as to analyze the growth rate of mycelia, biological efficiency, characters of fruiting body, proportion of high-quality mushrooms and input-output ratio of each strain. The results showed that the strain of Xiu 57 had the highest biological efficiency (62.53%), followed by Taixiu P86 (61.82%), and there was no significant difference between them. In terms of the proportion of high-quality mushrooms, the proportion of mushroom A of Xiu 57 and Taixiu P86 strains was both higher than 70%, which was obviously higher than that of other strains. According to the economic benefit analysis, the profit per bag of Xiu 57 and Taixiu P86 strains was 1.13 yuan and 1.08 yuan, respectively, and their input-output ratio was both greater than 1.30 yuan. Based on the above analysis, the strains of Xiu 57 and Taixiu P86 performed well among the tested strains, it was superior in the indexes such as the yield, characters and proportion of high-quality mushrooms, which were suitable for the cultivation of Wokou fungus sack in Luoyuan area.