茶多酚抗菌活性及其在水果复合保鲜剂中的应用

    Antimicrobial Activity of Tea Polyphenols and Its Application in Fruit Compound Preservative

    • 摘要: 利用茶多酚具有抗氧化、抑菌、绿色环保等特点,开发一款含茶多酚高效的天然水果复合保鲜剂。通过比较茶多酚、苯甲酸钠、山梨酸钾的抑菌效果,确定茶多酚的抑菌浓度;并进一步研究了茶多酚与被膜剂(巴西棕榈蜡和商业化被膜剂)复合保鲜剂在柑橘保鲜中的作用。抑菌试验结果表明,茶多酚对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌的最低抑菌浓度分别为0.4、0.6和1.0 g·L-1,其抑制细菌效果显著优于苯甲酸钠和山梨酸钾;而茶多酚对酿酒酵母无抑制作用。基于抑菌试验结果,复合保鲜剂中的茶多酚添加浓度确定为0.8 g·L-1。柑橘保鲜试验的结果显示,与空白对照相比,商业化被膜剂(CP)、商业化被膜剂+茶多酚(CP-TP)、巴西棕榈蜡(ZL)、巴西棕榈蜡+茶多酚(ZL-TP)处理均具有保鲜效果。含巴西棕榈蜡的处理的柑橘硬度和保水能力显著高于含商业化被膜剂的处理;ZL处理的果皮抑菌率和维生素C含量均显著高于CP处理;茶多酚能显著提高巴西棕榈蜡保鲜剂的抑菌率,维持维生素C含量和降低水果呼吸速率。研究结果表明棕榈蜡+茶多酚复合涂剂的保鲜效果最优。

       

      Abstract: Because tea polyphenols had the characteristics of anti-oxidation, bacteriostasis and environmental protection, an efficient natural fruit compound preservative containing tea polyphenols was developed. The inhibitory concentration of tea polyphenols was determined by comparing the antibacterial effects of tea polyphenols, sodium benzoate and potassium sorbate. Then, the effects of the compound preservative, containing tea polyphenols and coating agent (carnauba wax and commercial coating agent) on the preservation of Citrus reticulata were further studied. The bacteriostasis test results showed that the minimum inhibitory concentrations of tea polyphenols against Escherichiacoli, Bacillus subtilis and Staphylococcus aureus were 0.4 g·L-1, 0.6 g·L-1 and 1.0 g·L-1, respectively. The antibacterial effect of tea polyphenols was significantly better than that of sodium benzoate and potassium sorbate, but it had no inhibitory effect on Saccharomyces cerevisiae. Based on the bacteriostasis test results, the concentration of tea polyphenols in the compound preservative was determined to be 0.8 g·L-1. The preservation experimental results of Citrus reticulata showed that compared with the blank control, the treatments of commercial coating agent (CP), commercial coating agent+tea polyphenol (CP-TP), carnauba wax (ZL), carnauba wax+tea polyphenol (ZL-TP) all had the preservation effects. The hardness and water retention capacity of Citrus reticulata treated with carnauba wax were significantly higher than those treated with the commercial coating agent, and the bacteriostasis rate and vitamin C content of peel treated with carnauba wax were significantly higher than those treated with the commercial coating agent. Tea polyphenols could significantly improve the bacteriostasis rate of carnauba wax preservative, maintain the content of vitamin C and reduce the respiration rate of fruits. The results showed that the compound preservative of carnauba wax and tea polyphenol had the best preservation effect.

       

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