Abstract: RR-TZF gene plays an important role in plant growth, development and stress responses. OsTZF9 gene is a member of rice RR-TZF gene family. In order to analyze the biological function of OsTZF9 gene, a plant overexpression vector pCXUN-TZF9 driven by Ubiqutin promoter was constructed, and 6 clones of transgenic regenerated plants were obtained by using the agrobacterium-mediated method. Further analysis of the phenotype of the progenies of the transgenic plants showed that plant height, tiller number, effective panicle number, panicle length and 1000-grain weight of the OsTZF9-overexpressed plants were decreased compared with those of the wild-type Nipponbare; compared with the transgenic negative isolated plants, heading date of the OsTZF9-overexpressed plants was delayed.