Abstract: In order to explore the effects of four kinds of rice herbicides on the growth, yield and quality of tobacco leaves, according to the weed-controlling experimental method of herbicides in tobacco fields, four herbicides were sprayed on the soil surface of tobacco border check and covered with films 7 days before the transplanting of tobacco. The growth of tobacco plants was observed and recorded in different growth periods by means of the investigation and measurement method of tobacco agronomic traits. The contents of nicotine, total sugar, reducing sugar, total nitrogen, potassium and chlorine in tobacco leaves were determined by using Fourier near-infrared spectroscopy. The results showed that the application of the four herbicides delayed the small opening, spherical plant stage and mature period of tobacco by 0-6 days. The leaf area coefficients respectively decreased by 0.08-0.369 and 0.187-0.509 at the spherical plant stage and before harvesting & roasting. The effective number of leaves and plant height were slightly lower than those in the control treatment with clean water (CK), especially spraying 25 g·L^-1 penoxsulam. The yield of tobacco leaves treated with four herbicides did not reach a significant level compared with the control treatment with clean water(CK), but the proportion of medium tobacco was significantly higher than the control treatment with clean water (CK). The contents of nicotine, total nitrogen, potassium and chlorine in tobacco leaves of B2F and C3F treated with the four herbicides were slightly lower than those treated with clean water (CK), and the content of total sugar was slightly higher than that in the control treatment with clean water (CK). The contents of nicotine, total sugar and potassium in tobacco leaves of X2F were higher than those in CK, while the contents of total nitrogen and chlorine were slightly lower than those in CK. The above conclusions indicated that the dosages of four kinds of rice herbicides had little effect on the growth and quality of tobacco leaves when applied 7 days before the transplanting of tobacco plants, which could be used to control the weeds in tobacco fields and their previous rice fields.