Abstract: The rice blast, caused by Magnaporthe oryzae, is a main disease on rice production in China and even in the world. At present, the control methods including the cultivation management, chemical agents and the selection of disease-resistant varieties were mainly used in production to prevent and control the rice blast. Among them, the breeding of the disease-resistant varieties with disease-resistant genes has been proved to be the most economical effective and environmentally friendly choice. In this paper, the research work of our team in the screening, identification and application of resistance genes and the analysis of resistance mechanisms by using the techniques such as molecular biology and multi-omics analysis in the past 10 years were reviewed. In addition, the research progress of rice resistance genes and the avirulence genes of Magnaporthe oryzae in recent years were summarized, as well as the substances and metabolic pathways related to the resistance gene Piz-t, such as 5-hydroxytryptamine and tryptophan pathway. These information would be expected to provide reference for the molecular design of the disease-resistant breading in rice.