Abstract: The contents of polyphenols in tobacco leaves from different producing areas, different varieties and different parts in the tobacco area of Fujian were analyzed, in order to provide data reference for the production and quality evaluation of tobacco leaves. The contents of seven polyphenols including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, scopoletin, rutin and kaempferoside in 72 tobacco samples from X2F, C3F and B2F of CB-1, Yunyan 87 and K326 in 12 counties of three producing areas in Fujian from 2020 to 2021 were determined and analyzed by high performance liquid chromatography (HPLC-DAD). Then, the distribution of polyphenols in tobacco leaves from different producing areas, different varieties and different parts were compared. The results showed that the contents of chlorogenic acid and rutin accounted for 75%- 85% of the polyphenols in tobacco leaves in Fujian. There were great differences in the contents of polyphenols in tobacco leaves from different producing areas, different varieties and different parts. Among the main cultivated varieties in the tobacco area of Fujian, the total content of polyphenols in CB-1 was higher than that of K326 and Yunyan 87. There was a significant positive correlation (R²=0.948 6) between the total content of polyphenols in the three varieties and the sensory evaluation score of the unblended cigarette. The higher the total content of polyphenols, the higher the evaluation score of quality characteristics in sensory evaluation awarded.