Abstract: The regional test of new summer soybean varieties in Fujian in 2021 was carried out in the breeding field for fine varieties in Zhangping City. There were 12 tested varieties and Lvxie was used as the control group. And the agronomic characters, high yield, stress resistance and adaptability of the tested summer soybean varieties were comprehensively compared. The results showed that the yields of Huaxia No.26, Fuxiadou No.5 and Nannong 99-6 were higher than that of the control group Lvxie, while the yields of Quandou 24 and Fuxiadou No.4 were lower than that of the control group Lvxie, and the yield reduction reached the extremely significant level. Huaxia No.26 and Fuxiadou No.5 had good comprehensive performance, good commodity, strong resistance and wide adaptability. Nannong 99-6 and Fuxiadou No.1 had moderate growth period, and the main stems were pod-setting, which were suitable for close planting, with the potential for increasing yield and good resistance to stress. The plant traits of Huaxia No.18, Quandou 23, Funong No.6, Funong No.5 and Nannong 201 showed normal comprehensive performance, while the plant traits of Quandou 24 and Fuxiadou No.4 showed general comprehensive performance. According to the identification result of the resistance to anthracnose of the tested varieties, Nannong 99-6, Fuxiadou No.1 and Fuxiadou No.5 were disease-resistant (R) varieties, Quantou 24 was highly susceptible (HS) variety, Nannong 201 was susceptible (S) variety, and Fuxiadou No.4 was moderate susceptible (MS) variety. Therefore, after the comprehensive comparative analysis, it was suggested that Nannong 99-6 and Fuxiadou No.1 should be put into the production test next year. Huaxia No.26 and Fuxiadou No.5 would continue to participate in the regional trials next year. Huaxia No.18, Quandou 23, Funong No.6, Funong No.5 and Nannong 201 could continue to be observed and planted next year, while the test on Quantou 24 and Fuxiadou No.4 should be terminated.