紫玉兰花挥发油成分鉴定及其生物活性分析

孙娅; 解修超; 徐易洁; 邓百万

doi:10.13651/j.cnki.fjnykj.2023.01.003

紫玉兰花挥发油成分鉴定及其生物活性分析

孙娅¹,
解修超^1,2,3,4,
徐易洁¹,
邓百万^1,2,3,4

1. 陕西理工大学, 陕西汉中 723001;
2. 陕西省资源生物重点实验室, 陕西汉中 723001;
3. 陕南秦巴山区生物资源综合开发协同创新中心, 陕西汉中 723001;
4. 陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室, 陕西汉中 723001

基金项目:

陕西省教育厅重点科研计划项目(22JY016、22JK0320)

陕西省中央引导地方科技发展专项资金计划项目(2021ZY2-JD-09)。

详细信息

作者简介:
孙娅,女,1997年生,在读硕士研究生,主要从事微生物活性代谢产物研究。

中图分类号: S685.15
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出版历程
- 收稿日期: 2022-12-01
- 刊出日期: 2023-01-27

Component Identification and Biological Activity Analysis of the Volatile Oil from the Flowers of Magnolia liliflora

SUN Ya¹,
XIE Xiu-chao^1,2,3,4,
XU Yi-jie¹,
DENG Bai-wan^1,2,3,4

1. Shaanxi University of Technology, Hanzhong, Shaanxi 723001, China;
2. Shaanxi Province Key Laboratory of Biological Resources, Hanzhong, Shaanxi 723001, China;
3. Qinba Mountain Area Collaborative Innovation Center of Biological Resources Comprehensive Development in Shaanxi Province, Hanzhong, Shaanxi 723001, China;
4. Qinba State Key Laboratory of Biological Resources and Ecological Environment (Breeding Base) Built by Province and Ministry, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, China

摘要

摘要: 为提取紫玉兰花朵中的挥发油,分析其挥发油中的组分,探讨其挥发油的生物活性,为开发利用这一资源提供科学依据。采用正己烷萃取法提取紫玉兰花的挥发油,利用气相色谱-质谱联用技术(GC-MS)分离和鉴定其化学成分,并通过滤纸片扩散法和MTT法初步研究挥发油的抑菌活性和抗肿瘤活性。结果表明:从紫玉兰花的挥发油中共鉴定出46种化学成分,占总挥发油含量的92%,主要为烯烃类(17种,16.9%)、烷烃类(8种,18.43%)、酯类(6种,3.46%)、酚醇类(6种,4.18%)、醛酮类(4种,1.9%)、杂环类(1种,9.26%)醚类(1种,1.09%)及其他类别(3种,3.76%),其中含量较高的组分是加尔加拉文(二苯基四氢呋喃衍生物),相对含量达9.26%,其次是烷烃类的二十六烷、二十四烷以及正二十三烷,其相对含量分别为5.05%、4.57%和3.28%,余下物质的相对含量均低于2.5%。活性检测表明挥发油样品对枯草芽孢杆菌Bacillus subtilis 、大肠杆菌Escherichia coli、沙门氏菌Salmonella typhl、金黄色葡萄球菌Staphylococcus aureus均有抑菌活性,当挥发油浓度为1 000.0 μg·mL^-1时,对枯草芽孢杆菌抑菌圈直径达14.8 mm;对人早幼粒急性白血病细胞HL-60显示出较强的抗肿瘤活性,其IC₅₀达到84.3 μg·mL^-1。
- 紫玉兰花 /
- 挥发油 /
- GC-MS /
- 抑菌活性 /
- 抗肿瘤活性
Abstract: This paper aimed to extract the volatile oil from the flowers of Magnolia liliflora, analyze the components of the volatile oil, and explore the biological activity of the volatile oil, in order to provide scientific basis for the development and utilization of this resource. The volatile oil from the flowers of Magnolia liliflora was extracted by using the n-hexane extraction, and its chemical constituents were isolated and identified by gas chromatography-mass spectrometry (GC-MS). Then, the antibacterial activity and antitumor activity of the volatile oil were preliminarily studied through the filter diffusion method and MTT method. The results showed that: A total of 46 chemical components were identified from the volatile oil of Magnolia liliflora flowers, accounting for 92% of the total content of volatile oil, which were mainly alkenes (17 kinds, accounting for 16.9%), hydrocarbon (8 kinds, accounting for 18.43%), esters (6 kinds, accounting for 3.46%), phenolic alcohol (6 kinds, accounting for 4.18%), aldehydes (4 kinds, accounting for 1.9%), heterocyclics (1 kind, accounting for 9.26%), ethers (1 kinds, accounting for 1.09%) and other categories (3 kinds, accounting for 3.76%), among which the component with a relatively high content was Galgaravin (diphenyl tetrahydrofuran derivatives) with a relative content of 9.26%, followed by hexacosane, tetracosane and n-tricosane with the relative contents of 5.05%, 4.57% and 3.28%, respectively. The relative contents of the remaining substances were all lower than 2.5%. The activity detection showed that the volatile oil samples all had antibacterial activity against Bacillus subtili, Escherichia coli, Salmonella typhl and Staphylococcus aureus. When the concentration of volatile oil was 1000.0 μg·mL^-1, the inhibition zone diameter against Bacillus subtilis was 14.8 mm. It showed stronger antitumor activity against the human acute promyelocytic leukemia cell line HL-60 with IC₅₀ of 84.3 μg·mL^-1.
- Flowers of Magnolia liliflora /
- Volatile oil /
- GC-MS /
- Antibacterial activity /
- Antitumor activity

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