Abstract: In order to explore the application effect of different light quality as the light source of tobacco growth, by taking Nicotiana benthamiana as the experimental material under the condition of artificial climate chamber, three light sources with different light quality, including LED-v1 (R1.2/B1, R3.4/FR1), LED-v2 (R7.4/B1, R2.9/FR1), and LED-v1/v2 (R2.3/B1, R3.1/FR1), were set up to analyze the response of tobacco growth indicators to different light quality. The results showed that the plant height, stem thick, leaf area, blade number and biomass of Nicotiana benthamiana treated with LED-v1/v2 were significantly better than those of other treatments. Low R/FR and low R/B light quality could increase the N content in the leaves of tobacco, help to synthesize the organic matters and improve the stress resistance, and also improve the economic quality of tobacco. Appropriately reducing the R/B and R/FR values of LED-v1/v2 light quality was more conducive to the growth of f tobacco and its quality improvement. There was no significant difference in the chlorophyll fluorescence parameters such as photosynthetic reaction centre Ⅱ (ΦPSⅡ), apparent electron transport rate (ETR), PSⅡ maximal photochemical efficiency (Fv/Fm) among different treatments. In summary, the most suitable artificial light source for the growth of tobacco was LED-v1/v2 (R2.3/B1, R3.1/FR1).