Abstract: It was of great significance to clarify the laws and characteristics of nitrogen loss in tea plantation intercropping for scientifically evaluating the emission reduction effect of agricultural non-point source pollution in the ecological tea plantations. By taking the ecological tea plantation in Huanxi Town, Fuzhou City, Fujian Province as the research object, the long-term location experiment was carried out by using the runoff plot method. The volume of runoff from tea plantations under the conditions of monocropping along the downward slope (T1), intercropping along the downward slope (T2), monocropping in the terrace (T3) and intercropping in the terrace (T4) in 16 rainfall events from 2016 to 2018 was observed, in order to analyze the variation of nitrogen concentration and its loss amout in runoff. The intercropping plant was Paspalum notatum. The results showed that: according to the runoff volume in the downward slope (T1, T2) and terrace (T3, T4), the volume of runoff from tea plantations in intercropping was lower than that in monocropping. Along the downward slope, the TN concentration, AN concentration, NN concentration, TN loss amount, AN loss amount and NN loss amount in intercropping decreased by 32.0%, 44.4%, 23.8%, 38.5%, 49.8% and 30.8% compared with that in monocropping. In the terrace, the TN concentration, AN concentration, NN concentration, TN loss amount, AN loss amount and NN loss amount in intercropping decreased by 26.4%, 37.9%, 21.9%, 49.5%, 51.7% and 53.3% compared with that in monocropping. There was a significant correlation between the rainfall and runoff volume.