Effects of the Integrated Microbiome Agent on the Seed Germination andSeedling Growth of Watermelon and Its Control Effect Against Fusarium Wilt
-
摘要: 整合微生物组菌剂(简称整合菌剂)是以使用1年左右的微生物发酵床养猪垫料为基材生产的土壤修复剂,含有多个功能微生物组。通过采用滤纸萌发法,研究整合菌剂不同浸提液浓度对西瓜种子萌发的影响,结果表明:整合菌剂的浸提液对西瓜种子的萌发有明显抑制作用。添加量为5%,发芽率为53.33%,发芽指数(7.02)和种子活力指数(241.05)均显著低于两组对照(P＜0.05);胚根的生长(34.54 mm)与两组对照(34.56 mm和30.20 mm)无显著差异(P＜0.05)。随着添加量的增加,发芽率、胚根长、发芽指数、种子活力指数均呈下降趋势。通过盆栽试验,研究整合菌剂不同比例添加量对西瓜植株生长及枯萎病害的防治效果,结果表明:整合菌剂添加量10%,根长和株高较旧基质分别提高了40.82%和45.34%,促长效果最为显著(P＜0.05),对西瓜枯萎病防治效果达66.67%;整合添加量大于20%,根长和株高较旧基质分别降低了10.21%和3.97%,抑制作用显著(P＜0.05)。综合试验结果,建议整合菌剂可在西瓜定植时根部施用,添加量控制在10%以下,并结合防病药剂等其他措施才能达到对枯萎病的理想防效。Abstract: The integrated microbiome microbial agent (referred to as integrated microbial agent) was a soil remediation agent produced by using the pig litter of microbial fermentation bed for raising pigs for about one year as the base material, which contained multiple functional microbiomes. The effects of different concentrations of the integrated microbial agent extract on the germination of watermelon seeds were studied by using the filter paper germination method. The results showed that: the extract solution of the integrated microbial agent had significant inhibitory effect on the germination of watermelon seeds. When the addition amount was 5%, the germination rate was 53.33%, the germination index (7.02) and seed vigor index (241.05) were significantly lower than those of the two control groups (P＜0.05). There was no significant difference in the radicle growth (34.54 mm) between the two control groups (34.56 mm and 0.20 mm) (P＜0.05). With the increase of the addition amount, the germination rate, radicle length, germination index and seed vigor index showed a downward trend. Through the pot experiment, the control effects of different proportions of addition amount of the integrated microbial agents on the plant growth of watermelon and wilt disease were studied. The results showed that :The results showed that: when the addition amount of integrated microbial agent was 10%, the root length and plant height were increased by 40.82% and 45.34%, respectively, compared with the old substrate, and the growth promotion effect was the most significant (P＜0.05), and the control effect on watermelon fusarium wilt was 66.67%. When the addition amount of integrated microbial agent was more than 20%, the root length and plant height decreased by 10.21% and 3.97%, respectively, compared with the old substrate, and the inhibitory effect was significant (P＜0.05). According to the results of the experiment, it was suggested that the integrated microbial agent could be applied to the roots of watermelon during the planting process, and the addition amount should be controlled below 10%, Then, the ideal control effect on fusarium wilt could be achieved by combining with other measures such as the disease control agents.
-
Keywords:
- Integrated microbiome microbial agent /
- Watermelon /
- Seed germination /
- Fusarium wilt
-
-
[1] 刘双江,施文元,赵国屏.中国微生物组计划:机遇与挑战[J].中国科学院院刊,2017,32(3):241-250. [2] 朱永官,沈仁芳,贺纪正,等.中国土壤微生物组:进展与展望[J].中国农业文摘:农业工程,2018,3:6-12,38. [3] 朱永官,彭静静,韦中,等.土壤微生物组与土壤健康[J].中国科学:生命科学,2021,51(1):1-11. [4] BEI S,ZHANG Y,LI T,et al.Response of the soil microbial community to different fertilizer inputs in a wheat-maize rotation ona calcareous soil[J].Agric Ecosyst Environ,2018,260:58-69.
[5] GUO Z,HAN J,LI J,et al.Effects of long-term fertilization on soil organic carbon mineralization and microbial community structure[J].PLoS One,2019,14(1):e0216006.
[6] JIANG Y,LUAN L,HU K,et al.Trophic interactions as determinants of the arbuscular mycorrhizal fungal community with cascading plant promoting consequences[J].Microbiome,2020,8:142.
[7] FAN K,DELGADO-BAQUERIZO M,GUO X,et al.Biodiversity of key-stone phylotypes determines crop production in a 4-decade fertilization experiment[J].ISME J,2021,15(2):9550-561.
[8] YANG Y,WANG P,ZENG Z.Dynamics of bacterial communities in a 30-year fertilized paddy field under different organic-inorganic fertilization strategies[J].Agronomy,2019,9(1):14.
[9] 徐昭勇,胡海洋,许平, 等.人工合成微生物组的构建与应用[J].合成生物学,2021,2(2):181-193. [10] LIU Y X,QIN Y,BAI Y.Reductionist synthetic community approaches in root microbiome research[J].Current Opinion in Microbiology,2019,49:97-102.
[11] VORHOLT J A,VOGEL C, CARLSTROM C I,et al.Establishing causality:opportunities of synthetic communities for plant microbiome research[J].Cell Host & Microbe,2017,22(2):142-155.
[12] 刘波,陈倩倩,王阶平,等.整合微生物组菌剂的提出、研发与应用[J].中国农业科学, 2019,52(14):2450-2467. [13] 郑雪芳,刘波,朱育菁,等.整合微生物组菌剂对番茄种子萌发和幼苗生长的影响及其对青枯病的防治效果[J].中国生物防治学报,2019,35(6):908-914. [14] 须文,岑聪,徐彦军.不同基质配方对蔬菜种子萌发及幼苗生长的影响[J].江苏农业科学,2020,48(9):127-131. [15] 候立刚,刘会,芳田健,等.不同菌剂浓度对低温处理下水稻种子萌发能力的影响[J].种子,2021,3:87-90,96. [16] 黄志浩,王学虎,吴广利,等.微生物菌剂对玉米发芽及生长的影响[J].安徽农业科学,2022,50(21):28-30. [17] 蓝江林,刘波,吴小彬,等.2种不同养猪微生物发酵床垫料使用后的异质性分析[J].福建农业科技,2022,53(2):15-19. [18] 郑雪芳,刘波,朱育菁,等.设施番茄连作障碍土壤修复及其对青枯病害的防治效果[J].中国生物防治学报,2018,34(1):117-123. [19] 丁映风,朱红青,姚巧敏,等.复合微生物菌剂对设施栽培番茄的田间应用效果[J].云南农业科技,2023,1:11-13,19. [20] 黄书超,侯栋,颉建明,等.混合微生物菌剂对连作土壤中黄瓜幼苗生长的影响[J].甘肃农业大学学报,2021,56(2):83-89. [21] 杜少平,唐超男,马忠明,等.不同微生物菌剂对旱砂田西瓜生长和微生物区系的影响[J].寒旱农业科学,2022,1(1):57-61. [22] 徐伟慧,吕智航,史一然,等.西瓜复合根际促生菌剂构建与促生效应研究[J].浙江农业学报,2018,30(5):778-786. [23] 王夏雯,吴绍军,孟佳丽,等.2种类型微生物肥料对连作西瓜枯萎病的防治效果研究[J].江西农业学报,2019,31(11):23-28.
计量
- 文章访问数: 124
- HTML全文浏览量: 0
- PDF下载量: 16
下载:
