Abstract: The integrated microbiome microbial agent (referred to as integrated microbial agent) was a soil remediation agent produced by using the pig litter of microbial fermentation bed for raising pigs for about one year as the base material, which contained multiple functional microbiomes. The effects of different concentrations of the integrated microbial agent extract on the germination of watermelon seeds were studied by using the filter paper germination method. The results showed that: the extract solution of the integrated microbial agent had significant inhibitory effect on the germination of watermelon seeds. When the addition amount was 5%, the germination rate was 53.33%, the germination index (7.02) and seed vigor index (241.05) were significantly lower than those of the two control groups (P＜0.05). There was no significant difference in the radicle growth (34.54 mm) between the two control groups (34.56 mm and 0.20 mm) (P＜0.05). With the increase of the addition amount, the germination rate, radicle length, germination index and seed vigor index showed a downward trend. Through the pot experiment, the control effects of different proportions of addition amount of the integrated microbial agents on the plant growth of watermelon and wilt disease were studied. The results showed that :The results showed that: when the addition amount of integrated microbial agent was 10%, the root length and plant height were increased by 40.82% and 45.34%, respectively, compared with the old substrate, and the growth promotion effect was the most significant (P＜0.05), and the control effect on watermelon fusarium wilt was 66.67%. When the addition amount of integrated microbial agent was more than 20%, the root length and plant height decreased by 10.21% and 3.97%, respectively, compared with the old substrate, and the inhibitory effect was significant (P＜0.05). According to the results of the experiment, it was suggested that the integrated microbial agent could be applied to the roots of watermelon during the planting process, and the addition amount should be controlled below 10%, Then, the ideal control effect on fusarium wilt could be achieved by combining with other measures such as the disease control agents.