8个春石斛栽培种花瓣挥发性成分差异分析

孔兰; 叶秀仙; 林榕燕; 钟淮钦

doi:10.13651/j.cnki.fjnykj.2023.06.005

8个春石斛栽培种花瓣挥发性成分差异分析

福建省特色花卉工程技术研究中心/福建省农业科学院作物研究所, 福建福州 350013

基金项目:

福建省科技计划公益类项目(2021R1031005)

福建省农业科学院自由探索项目(ZYTS202205)

福建省种业创新与产业化工程(ZYCX-LY-202102)

福建省人民政府、中国农业科学院'5511’协同创新项目(XTCXGC2021016)

福建省农业科学院特色花卉科技创新团队建设项目(CXTD2021010-2)。

详细信息

作者简介:
孔兰,女,1987年生,助理研究员,主要从事兰科植物分子生物学研究。

通讯作者:
钟淮钦,男,1979年生,副研究员,主要从事观赏植物种质资源评价与创新利用研究(E-mail:zhqeast@163.com)。

中图分类号: S682.31
计量
- 文章访问数: 114
- HTML全文浏览量: 0
- PDF下载量: 18
出版历程
- 收稿日期: 2023-05-09
- 刊出日期: 2023-06-27

Difference Analysis of the Volatile Components in the Petals ofEight Cultivated Species of Dendrboium

Fujian Engineering Technology Research Center for Characteristic Floriculture/Crop Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 为研究不同春石斛栽培种花瓣中挥发性成分组成特点及其差异,采用顶空固相微萃取(HS-SPME)结合气相色谱-质谱(GC-MS)技术,分析了杂交紫花、黑毛珍珠、红爵士、火鸟公主、黄钻戒、台湾公主、大汉王后和耳环等8个春石斛栽培种花瓣挥发性成分及其相对含量,并应用主成分分析和聚类分析确定品种间的亲疏关系。结果表明:8个栽培种共检测到130种挥发性成分,以萜烯类化合物为主;春石斛花瓣中的主要挥发性成分为β-罗勒烯、β-石竹烯、芳樟醇、α-蒎烯、水杨酸甲酯和辛醇,因其组成和含量的变化导致了品种间的香气感官差异;主成分分析结果显示8个栽培种具有明显的区域分布特征。此外,聚类分析将8个栽培种分为3组:黑毛珍珠和杂交紫花为一组,火鸟公主、红爵士和黄钻戒为一组,台湾公主、大汉王后和耳环为一组。该研究结果为春石斛香气物质的利用和香型品种的培育提供了参考。
- 春石斛 /
- 挥发性成分 /
- HS-SPME/GC-MS /
- 萜烯类化合物 /
- 主成分分析
Abstract: In order to study the composition characteristics of volatile components in the petals of different cultivated species of Dendrboium and their differences, the headspace solid-phase microextraction (HS-SPME) combined with the gas chromatography-mass spectrometry (GC-MS) was used to analyze the volatile components and their relative contents in the petals of eight cultivated species of Dendrboium, including Zajiaozihua, Heimaozhenzhu, Hongjueshi, Huoniaogongzhu, Huangzuanjie, Taiwangongzhu, Dahanwanghou and Erhuan. And the principal component analysis and cluster analysis were used to determine the relationship between the varieties. The results showed that a total of 130 volatile components were detected in the eight cultivated species, mainly terpenoids. The main volatile components in the petals of Dendrboium were β-ocimene, β-caryophyllene, linalool, α-pinene, methyl salicylate and octyl alcohol. The changes of their composition and content led to the difference of aroma sensory between the varieties. The results of principal component analysis showed that the eight cultivated species had obvious regional distribution characteristics. In addition, the eight cultivated species were divided into three groups by cluster analysis: Heimaozhenzhu and Zajiaozihua were a group; Huoniaogongzhu, Hongjueshi and Huangzuanjie were a group; Taiwangongzhu, Dahanwanghou and Erhuan were a group. The results of this study could provide reference for the utilization of aroma substances and the cultivation of aroma type varieties of Dendrboium.
- Dendrboium /
- Volatile components /
- HS-SPME/GC-MS /
- Terpenoids /
- Principal component analysis

HTML全文

参考文献(24)

[1]	潘丽晶,曹友培,肖杨,等.观赏石斛育种技术研究综述[J].广东农业科学,2009(9):71-73.
[2]	谢光明,李秀梅,刘进平.观赏石斛兰新品种朝阳和白雪[J].中国热带研究,2019,90(5):45-47.
[3]	张琼,曾燕楠,张宁宁,等.春石斛兰组培关键技术研究[J].天津农业科学,2009,15(5):75-77.
[4]	李伟倩.多花春石斛开花特性与快繁体系优化研究[D].北京:中国林业科学研究院,2018.
[5]	WANG S,DU Z H,YANG X Y,et al.An integrated analysis of metabolomics and transcriptomics reveals significant differences in floral scents and related gene expression between two varieties of Dendrobium loddigesii[J]. Applied Science,2022,12:1262.
[6]	杨慧君,姚娜,李潞滨,等.建兰花香成分的GC-MS分析[J].中国农学通报,2011,27(16):104-109.
[7]	BERA P,MUKHERJEE C,MITRA A.Enzymatic production and emission of floral scent volatiles in Jasminum sambac[J].Plant Science,2017,256:25-38.
[8]	DU F,WANG T,FAN J M,et al.Volatile composition and classification of Lilium flower aroma types and identification, polymorphisms, and alternative splicing of their monoterpene synthase genes[J].Horticulture Research,2019,6:110.
[9]	ABBAS F,ZHOU Y,HE J,et al.Metabolite and transcriptome profiling analysis revealed that melatonin positively regulates floral scent production in Hedychium coronarium[J].Frontiers Plant Science,2021,12:808899.
[10]	LO M M,BENFODDA Z,BÉNIMÉLIS D,et al.Extraction and identification of volatile organic compounds emitted by fragrant flowers of three Tillandsia species by HS-SPME/GC-MS[J].Metabolites,2021,11:594.
[11]	YANG G X,QIN Y,JIA Y H,et al.Transcriptomic and metabolomic data reveal key genes that are involved in the phenylpropanoid pathway and regulate the floral fragrance of Rhododendron fortunei[J].BMC Plant Biology,2023,23:8.
[12]	李崇晖,黄明忠,黄少华,等.4种石斛属植物花朵挥发性成分分析[J].热带亚热带植物学报,2015,23(4):454-462.
[13]	宋小蒙,王洪新,马朝阳,等.GC-S分析金钗石斛花挥发性成分[J].食品与生物技术学报,2019,38(9):133-138.
[14]	王元成,曾艺芸,李振坚,等.细叶石斛和翅梗石斛花朵赋香成分的GC-MS分析[J].林业科学研究,2020,33(3):116-123.
[15]	王元成,张萌,周晓星,等.基于GC-MS的五种石斛花朵挥发性成分鉴定与差异性分析[J].林业科学研究,2022,35(1):132-140.
[16]	DU Z H,JIN Y X,WANG W Z,et al.Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum[J].Frontiers Plant Science,2022,13:1030492.
[17]	DUDAREVA N,NEGRE F,NAGEGOEDA D A,et al.Plant volatiles: recent advances and future perspectives[J].Critical Reviews in Plant Sciences,2006,25:417-440.
[18]	LI N H,DONG Y X,LV M,et al.Combined analysis of volatile terpenoid metabolism and transcriptome reveals transcription factors related to terpene synthase in two cultivars of Dendrobium officinale flowers[J].Frontiers in Genetics,2021,12:661296.
[19]	颜沛沛,叶炜,江金兰.报春石斛花香气成分及其日变化规律[J].亚热带植物科学,2020,49(3):168-174.
[20]	曹桦,许凤,陆琳,等.4种香花型石斛花朵挥发性成分GC-MS分析[J].中国农学通报,2021,37(13):56-62.
[21]	LI J J,YU X J,SHAN Q R,et al.Integrated volatile metabolomic and transcriptomic analysis provides insights into the regulation of floral scents between two contrasting varieties of Lonicera japonica[J].Frontiers Plant Science,2022,13:989036.
[22]	陈艺荃,林兵,钟淮钦,等.不同杂交兰品种花朵挥发性成分分析[J].中国细胞生物学学报,2019,41(10):1901-1908.
[23]	BEAK Y S,RAMYA M,AN H R,et al.Volatiles profile of the floral organs of a new hybrid Cymbidium,'Sunny Bell' using headspace solid-phase microextraction gas chromatography-mass spectrometry analysis[J].Plants,2019,8:251.
[24]	丁星文,王珍珍,王其刚,等.不同花色滇牡丹花香成分分析[J].南方园艺,2022,33(3):25-30.