Identification and Evaluation of the Low-temperature Germination Ability of 52 Bitter Gourd (Momordica charantia) Germplasm Seeds
-
摘要: 为评价苦瓜种子的低温发芽能力,测定了52份苦瓜种质种子在15℃处理下的8个发芽指标,并运用因子分析、相关分析、主成分分析、隶属函数分析、聚类分析、回归分析等方法进行综合性评价。结果表明:(1)52份苦瓜种质种子的发芽率、发芽势、生长势、发芽指数、活力指数、平均发芽时间、平均发芽速度和种子萌发指数均出现不同程度的差异,变异幅度为25.648%～127.182%。(2)发芽率、发芽势、生长势、发芽指数、活力指数、种子萌发指数两两之间呈极显著正相关,平均发芽速度与其他所有的指标均呈极显著负相关。(3)主成分分析共提取到两个主成分,累积贡献率为87.773%,其中主成分1贡献率为71.313%,主成分2贡献率为16.460%。(4)聚类分析将参试材料分成4个类型,基于综合评价值排序发现BG9、BG5、BG15、BG8和BG55的低温发芽能力强。(5)建立低温发芽能力评价方程D=0.162+0.001 ×X1+0.003×X2+0.137×X3+0.02×X5,为挖掘及培育耐冷品种提供科学依据,也为简化生产流程、提高生产效率提供理论参考。Abstract: In order to evaluate the germination ability of bitter gourd seeds at low temperature, 8 germination indexes of 52 bitter gourd germplasm seeds were measured at 15℃, and the ability was comprehensively evaluated by using the factor analysis, correlation analysis, principal component analysis, membership function analysis, cluster analysis and regression analysis. The results showed that: (1) The germination rate, germination potential, growth potential, germination index, vigor index, average germination time, average germination speed and seed germination index of 52 bitter gourd germplasm seeds all showed different degrees of differences, and the variation ranged from 25.648% to 127.182%. (2) The germination rate, germination potential, growth potential, germination index, vigor index and seed germination index were significantly positively correlated with each other, while the average germination speed was significantly negatively correlated with all other indexes. (3) Two principal components were extracted by principal component analysis, and the cumulative contribution rate was 87.773%, of which the contribution rate of principal component 1 was 71.313% and the contribution rate of principal component 2 was 16.460%. (4) The cluster analysis divided the tested materials into four categories, and based on the comprehensive evaluation value, it was found that BG9, BG5, BG15,BG8 and BG55 had strong germination ability under low temperature. (5) The evaluation equation D=0.162+0.001 ×X1+0.003×X2+0.137×X3+0.02×X5 of low-temperature germination ability was established, which provided scientific basis for excavating and cultivating cold-resistant varieties, and also provided theoretical reference for simplifying the production process and improving the production efficiency.
-
-
[1] 袁廷庆,符启位,刘成平,等.浸种时间、催芽温度及药剂处理对苦瓜种子发芽的影响[J].热带农业科学, 2018, 38(6): 9-12. [2] 孙奉良.苦瓜耐低温性指标鉴定与筛选[D].武汉: 华中农业大学, 2003. [3] 牛玉, 刘子记, 刘昭华, 等.苦瓜种质资源耐冷性鉴定[J].北方园艺, 2017(24):1-6. [4] 张瑜,严琳玲,罗小燕, 等.4种柱花草种子发育研究[J].种子,2016,35(1):30-34,37. [5] 马鸿艳,栾非时,李愚鹤.不同温度环境对黄瓜生长、产量、理化特性的影响[J].东北农业大学学报,2004, (6):697-700. [6] 位明明,李维国,高新生, 等.中国热带作物抗寒育种研究进展与展望[J].热带作物学报,2015,36(4):821-828. [7] 刘宏宇, 姚雪, 栾非时, 等.厚皮甜瓜种子萌芽期耐冷性评价方法研究[J].东北农业大学学报, 2016, 47(7): 24-31. [8] 王聪艳, 王彬, 张肖, 等.温度对西瓜种子发芽的影响[J].安徽农业科学, 2009, 37(23): 10953-10954. [9] 黄斌, 李文科, 孙敏涛, 等.复硝酚钠对低温下黄瓜种子萌发和幼苗耐寒性的影响[J].核农学报, 2022, 36(4): 845-855. [10] WEIMER T C.Estimates of heritabilities and variance components for low-temperature germination ability in cucumber[J]. Journal of the American Society for Horticultural Science, 1984, 109(5): 664-667.
[11] 王红飞.黄瓜种质资源低温耐受性的鉴定评价及QTL初步定位研究[D].北京: 中国农业科学院, 2014: 12-25. [12] SHARIFI P.Evaluation on sixty-eight rice germplasms in cold tolerance at germination stage[J].Rice Science, 2010, 17(1): 77-81.
[13] WANG L J, ZHANG P, WANG R N, et al.Effects of variety and chemical regulators on cold tolerance during maize germination[J].Journal of Integrative Agriculture,2018, 17(12):2662-2669.
[14] REEDR C, BRADFORD K J, KHANDA Y I.Seed germination and vigor: ensuring crop sustainability in a changing climate[J].Heredity,2022,128:450-459.
[15] DEMIR I, ERMIS S, MAVI K, et a1.Mean germination time of pepper seed lots(Capsicum annuum L.) predicts size and uniformity of seedlings in germination tests and transplant modules[J].Seed Science and Technology, 2008, 36(1): 21-30.
[16] 沈镝, 李锡香.苦瓜种质资源描述规范和数据标准[M].北京:中国农业出版社,2008. [17] 张慧静,庞胜群,吉雪花, 等.不同制干辣椒品种种子萌发期抗逆性评价[J].北方园艺,2019(22):1-7. [18] 胡晋, 王建成.种子检验技术[M].北京: 中国农业大学出版社, 2016. [19] 苗永美, 宁宇, 曹玉杰, 等.黄瓜萌芽期和苗期耐冷性评价[J].应用生态学报,2013,24(7):1914-1922. [20] 郑昀晔, 曹栋栋, 张胜, 等.多胺对玉米种子吸胀期间耐冷性和种子发芽能力的影响[J].作物学报, 2008, 34(2): 261-267. [21] 李志博, 魏亦农.北疆主栽棉花种子对渗透胁迫的响应及其萌发力差异评价[J].种子, 2010, 29(7): 1-4. [22] 徐建伟, 张晨, 曾晓燕, 等.近十年新疆北疆主栽棉花种子低温萌发能力差异评价[J].新疆农业科学, 2017, 54(9): 1569-1578. [23] 黄海涛, 胡江, 徐冬梅, 等.豇豆萌芽期耐冷性综合鉴定与评价[J].西北农业学报, 2019, 28(2): 237-246. [24] 韩敏, 李明明, 陈晨, 等.番茄砧木种子萌芽期与幼苗期耐冷性关系[J].生态学杂志, 2018, 37(3): 884-890. [25] 黄海涛.长豇豆耐冷种质资源筛选、遗传图谱构建及结荚期耐冷性QTL定位[D].温江:四川农业大学, 2018. [26] 王冲.西瓜嫁接耐低温砧木筛选及嫁接苗耐低温的生理基础研究[D].沈阳: 沈阳农业大学, 2020. [27] 常瑶.聚乙二醇和蛭石引发对不结球白菜种子萌发和幼苗生长的影响[D].南京: 南京农业大学, 2013. [28] 赵波, 庞胜群, 陈恺, 等.加工番茄种子发芽及苗期耐冷性差异研究[J].中国瓜菜, 2007 (3): 7-9. [29] 陈小凤, 黄如葵, 冯诚诚, 等.苦瓜芽期耐冷性鉴定与评价[J].种子, 2017, 36(1): 36-39. [30] 姚雪.甜瓜种质资源耐冷性的鉴定评价及其关联分析研究[D].哈尔滨: 东北农业大学, 2016. [31] 张陇艳,程功敏,魏恒玲,等.陆地棉种子萌发期对低温胁迫的响应及耐冷性鉴定[J].中国农业科学,2021,54(1):19-33. [32] 陈娜, 郭鸿韦, 许静, 等.发芽期与出苗期试验相结合筛选耐低温花生[J].花生学报, 2022, 51(4): 44-50,69.
计量
- 文章访问数: 1615
- HTML全文浏览量: 0
- PDF下载量: 31
下载:
