Abstract: In order to evaluate the germination ability of bitter gourd seeds at low temperature, 8 germination indexes of 52 bitter gourd germplasm seeds were measured at 15℃, and the ability was comprehensively evaluated by using the factor analysis, correlation analysis, principal component analysis, membership function analysis, cluster analysis and regression analysis. The results showed that: (1) The germination rate, germination potential, growth potential, germination index, vigor index, average germination time, average germination speed and seed germination index of 52 bitter gourd germplasm seeds all showed different degrees of differences, and the variation ranged from 25.648% to 127.182%. (2) The germination rate, germination potential, growth potential, germination index, vigor index and seed germination index were significantly positively correlated with each other, while the average germination speed was significantly negatively correlated with all other indexes. (3) Two principal components were extracted by principal component analysis, and the cumulative contribution rate was 87.773%, of which the contribution rate of principal component 1 was 71.313% and the contribution rate of principal component 2 was 16.460%. (4) The cluster analysis divided the tested materials into four categories, and based on the comprehensive evaluation value, it was found that BG9, BG5, BG15,BG8 and BG55 had strong germination ability under low temperature. (5) The evaluation equation D=0.162+0.001 ×X₁+0.003×X₂+0.137×X₃+0.02×X₅ of low-temperature germination ability was established, which provided scientific basis for excavating and cultivating cold-resistant varieties, and also provided theoretical reference for simplifying the production process and improving the production efficiency.