米口袋染色体制片技术优化及其核型分析

李玉萍; 欧香; 苗淑杏; 朱士农

doi:10.13651/j.cnki.fjnykj.2023.08.006

米口袋染色体制片技术优化及其核型分析

金陵科技学院园艺园林学院, 江苏南京 210038

基金项目:

南京市绿化园林局科技项目(YLKJ202111JH)。

详细信息

作者简介:
李玉萍,女,1976年生,博士,副教授,主要从事园林植物遗传育种和应用研究。

通讯作者:
朱士农,男,1963年生,博士,教授,主要从事园艺植物栽培、新品种选育的研究(E-mail:zsn@ jit.edu.cn)。

中图分类号: R284.2
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出版历程
- 收稿日期: 2023-07-19
- 刊出日期: 2023-08-27

Optimization of the Chromosome Production Technology of Gueldenstaedtia verna and Its Karyotype Analysis

College of Horticulture & Landscape, Jinling Institute of Technology, Nanjing, Jiangsu 210038, China

摘要

摘要: 以米口袋根尖为供试材料,用常规压片法探讨不同预处理方法和预处理时间对染色体制片的影响,优化染色体制片技术,并对其进行染色体核型分析,旨在为米口袋的分类、种质资源保存和利用及育种提供细胞学依据。结果表明:(1)当预处理时间为3 h时,0.1%秋水仙素溶液预处理效果最好;当预处理时间为6 h时,0.01%秋水仙素溶液预处理效果最好。得到的分裂相多,染色体浓缩程度适宜,分散性较好,形态清晰;(2)米口袋体细胞染色体数目为2n=2x=14,为二倍体植物。核型公式K(2n)=2x=14=10m+4sm,具中部着丝点染色体(m)的为第1、2、3、4、7对染色体,具近中部着丝点染色体(sm)的为第4、5对染色体。染色体相对长度组成为2n=14=2L+8M2+ 4M1,染色体长度比1.30,没有臂比大于2的染色体,属“1A”类型,为对称型核型,说明米口袋在进化上属于比较原始的类型。
- 米口袋 /
- 染色体 /
- 核型分析 /
- 制片技术
Abstract: The effects of different pretreatment methods and pretreatment time on the chromosome production were explored by using the conventional tabletting method with Gueldenstaedtia verna as the tested material, in order to optimize the chromosome production technology. And the chromosome karyotype analysis was carried out to provide the cytological basis for the classification of Gueldenstaedtia verna, the preservation & utilization of germplasm resources and its breeding. The results showed that: (1) When the pretreatment time was 3 h, the pretreatment effect of 0.1% colchicine solution was the best, while when the pretreatment time was 6 h, the pretreatment effect of 0.01% colchicine solution was the best. There were many division phases, the degree of chromosome condensation was suitable, the dispersion was better, and the morphology was clear. (2) The chromosome number of somatic cells of Gueldenstaedtia verna was 2n=2x=14, which was a diploid plant. The karyotype formula was K(2n)=2x=14=10m+4sm. The chromosomes with the metacentric chromosome (m) were the 1st, 2nd, 3rd, 4th and 7th pairs of chromosomes, and the chromosomes with the submetacentric chromosome (sm) were the 4th and 5th pairs of chromosomes. The composition of the relative length of chromosome was 2n=14=2L+8M2+4M1, the length ratio of chromosome was 1.30, and there were no chromosomes with the arm ratios greater than 2, which belonged to the ″1A″ type and was a symmetrical karyotype, indicating that the Gueldenstaedtia verna was a relatively original type in the evolution.
- Gueldenstaedtia verna /
- Chromosome /
- Karyotype analysis /
- Production technology

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