Abstract:
The chorismic acid synthetase
AroC is the key enzyme for the synthesis of aromatic compounds in the shikimic acid pathway. Identifying the characteristics of
aroC gene in
Brevibacillus brevis and its transcriptional regulators will lay a foundation for the study of the mechanism of action of
Brevibacillus brevis. The
aroC gene was cloned from
Brevibacillus brevis FJAT-0809-GLX, and then the bioinformatics analysis and the prediction of transcription factor were carried out. The sequence length of
aroC gene in
Brevibacillus brevis FJAT-0809-GLX was 1164 bp, and the GenBank accession number was OR475562. The
aroC gene sequence of
Brevibacillus brevis FJAT-0809-GLX had the highest homology with that of
Brevibacillus brevis NBRC 100599, which was 98.88%. The amino acid sequence of
AroC had the highest homology with
Brevibacillus brevis HNCS-1, which was 100%. Its encoded protein was a hydrophilic protein without signal peptide, which was located in the cytoplasm. A total of 23 transcription factors targeting
BbaroC were identified from 10 species, with 12 and 3 transcription factors predicted from
Escherichia coli (Strain K12 MG1655) and
Bacillus subtilis (Strain 168), respectively.
BbaroC may play an important role in the production of antibacterial active substances of
Brevibacillus brevis, which would provide basis for its wide application in agriculture.