Abstract: In order to explore new drug molecules with higher antiviral activity against plant viruses, 12 fluorinated isoquinolinedione derivatives were synthesized by using cheaper raw materials and milder reaction conditions, and the anti-Potato virus Y (PVY) activity of the synthesized target compounds was determined by using the half-leaf necrosis method. The results showed that the fluorinated isoquinolinedione derivatives had good inhibitory activity against potato virus Y (PVY). Among them, the compound 3e showed good effects in the therapeutic activity (43.5%), protective activity (47.1%) and inactivate activity (51.9%), which was comparable to the positive control agricultural antiviral drug ribavirin. It was expected that the compounds with higher antiviral activity could be obtained by some structural modification on the basis of its structure.