响应面法优化超声辅助提取烟叶废渣蛋白工艺条件

Optimization of Ultrasonic-assisted Extraction Process Conditions of Protein from Tobacco Waste Residue by Response Surface Methodology

摘要: 烟叶废渣提取蛋白是其综合利用途径之一，超声辅助碱提有助于获得更多的烟叶废渣蛋白。在单因素试验基础上，采用响应面法优化烟叶废渣超声辅助提取蛋白工艺。结果表明：当缓冲液pH为10、缓冲液体积为32.77 mL、超声温度为70.9℃、超声时间为46.1 min时，烟叶废渣的蛋白提取量达（19.13±0.21）mg·g⁻¹，相比优化前提高了140.6%，该工艺能为再造烟叶脱蛋白提供技术参考。

Abstract: The extraction of protein from tobacco waste residue is one of its comprehensive utilization ways. The ultrasonic-assisted alkaline extraction is helpful to obtain more protein from the waste residue tobacco leaves. On the basis of single factor experiment, the response surface method was used to optimize the ultrasonic-assisted extraction process of protein from the waste residue of tobacco leaves. The results showed that: when the pH of buffered solution was 10, the volume of buffered solution was 32.77 mL, the ultrasonic temperature was 70.9 °C, and the ultrasonic time was 46.1 min, the protein extraction amount of tobacco leaf waste residue reached (19.13 ± 0.21) mg·g⁻¹, which was 140.6 % higher than that before optimization. This process could provide technical reference for removing protein from the reconstituted tobacco leaves.