Abstract: In order to optimize the technology of increasing stress resistance and yield of cassava, five concentrations of rooting powder solution (0, 10.0, 20.0, 40.0, 60.0 mg·L⁻¹) for soaking seeds and two soaking time (6, 12 h) were set up by taking the non-soaking treatment as the control group (CK). Then, their effects on the agronomic traits, storage root yield and quality of cassava were studied. The results showed that: compared with no seed soaking, after soaking in the rooting powder solution with the concentration of 20.0, 60.0 mg·L⁻¹ for 6 h and soaking in the solution with the concentration of 20.0−60.0 mg·L⁻¹ for 12 h, the stem diameter of cassava was significantly increased. After soaking in the rooting powder solution with the concentration of 20.0 mg·L⁻¹ for 6 h, the length and diameter of storage root was significantly increased. After soaking in the rooting powder solution with the concentration of 0, 10.0, 60.0 mg·L⁻¹ for 12 h, the length of storage root was significantly increased. After soaking in the rooting powder solution with the concentration of 0, 40.0, 60.0 mg·L⁻¹ for 6 h and soaking in the solution with the concentration of 40.0, 60.0 mg·L⁻¹ for 12 h, the diameter of storage root was significantly increased. After soaking in the rooting powder solution with the concentration of 20.0 mg·L⁻¹ for 6 h, the fresh storage root yield was significantly increased by 42.3%, the starch yield was significantly increased by 49.9%, and the harvest index was significantly increased by 10.5%. In conclusion, soaking the seed stem of cassava with 20.0 mg·L⁻¹ rooting powder solution for 6 h was recommended as the yield-increasing technology.