姜黄素、β-胡萝卜素对太湖猪精液常温保存效果的影响

祁亚东; 梁佳玟; 侯振业; 冯宇辰; 赵孝杰; 吴怡; 吴结革; 刘京鸽

doi:10.13651/j.cnki.fjnykj.2024.07.004

姜黄素、β-胡萝卜素对太湖猪精液常温保存效果的影响

Exploration of Curcumin and β-Carotene on the Preservation Effect of Taihu Pig Semen at Room Temperature

摘要

摘要: 为了探究不同类别的植物源性抗氧化剂对太湖猪精液常温保存效果的影响，选取了姜黄素、β-胡萝卜素两种常见的抗氧化剂，分别以不同浓度（姜黄素：0、3、6、9 µg·mL⁻¹；β-胡萝卜素：0、10、20、40 µg·mL⁻¹）添加到太湖猪精液稀释液中，采用目测法、低渗肿胀法和瑞氏-吉姆萨染色法检测精子在17℃保存环境中第1、3、5、7 d的活力、质膜完整率和顶体完整率。结果表明：添加一定浓度的姜黄素、β-胡萝卜素均能显著提高精子质量，其中姜黄素的最适添加浓度为6 µg·mL⁻¹，β-胡萝卜素的最适添加浓度为20 µg·mL⁻¹。

Abstract: In order to explore the effect of different types of plant-derived antioxidants on the preservation of Taihu boar semen at room temperature, this experiment selected two commonly used antioxidants, curcumin and β-carotene. They were added at different concentrations（curcumin: 0, 3, 6, 9µg·mL⁻¹; β-carotene: 0, 10, 20, 40µg·mL⁻¹）to the diluted Taihu boar semen. Visual inspection, hypo-osmotic swelling test, and Wright-Giemsa staining method were employed to assess sperm vitality, plasma membrane integrity, and acrosome integrity on days 1, 3, 5, and 7 of preservation at 17°C. The results showed that the addition of certain concentrations of curcumin and β-carotene significantly improved sperm quality, with the optimal concentrations being 6 µg·mL⁻¹ for curcumin and 20 µg·mL⁻¹ for β-carotene.

HTML全文

参考文献(21)

施引文献

资源附件(0)