Abstract: The effects of cold storage on the postharvest fruit quality and active oxygen metabolism of Passiflora edulis Sims were discussed, in order to provide reference for the research and development of storage and preservation technology of Passiflora edulis after harvest. The changes of fruit quality, the production rate of superoxide anion free radical, malondialdehyde (MDA) content, peroxidase (POD) activity, superoxide dismutase (SOD) activity and catalase (CAT) activity of golden Passiflora edulis during the storage process at room temperature (25±1) ℃ and cold storage (4±1) ℃ were studied in this paper. The results showed that the indicators such as the total soluble solid, titratable acid, ascorbic acid, free amino acid of Passiflora edulis were relatively high and changed relatively slowly under the cold storage condition compared with the room temperature condition. The cold storage could effectively delay the degradation of soluble protein, and there was no significant difference in the solid-acid ratio between the two storage temperatures. The cold storage could inhibit the production of superoxide anion free radicals in Passiflora edulis fruit, and the changes of SOD, POD and CAT activities were relatively stable at low temperature. From the perspective of MDA content, under the condition of cold storage, the content of MDA was relatively low in the early stage of storage and higher in the later stage, but there was no significant difference between the treatments during the whole storage process. In summary, the cold storage could slow down the substance consumption such as sugar and acid in Passiflora edulis after harvest to a certain extent, so as to maintain the flavor and quality of Passiflora edulis. It could also inhibit the production of superoxide anion free radicals, delay the change of antioxidant enzyme activities, and improve the storeability of Passiflora edulis.