福建本地益智不同组织挥发油成分的GC-MS分析

    GC-MS Analysis of Volatile Oil Components in Different Fissues of Alpinia oxyphylla in Fujian

    • 摘要: 果实是益智的主要收获部位,而茎秆、叶片和块根通常都未被利用。为了充分利用这些资源,以福建本地益智鲜叶、茎秆、块根和鲜果为材料,采用水蒸气蒸馏法萃取其同一时间不同组织的挥发油,计算各组织挥发油的含量,并采用顶空进样法和气相色谱-质谱联用(GC-MS)技术对益智不同组织中挥发油成分进行分析。结果表明:福建本地益智不同组织均含有挥发油成分,其中块根中挥发油含量最高,达0.22%,其次是叶片,茎最少。益智不同组织挥发油成分共检出52种成分,发现烯烃类物质数量最多,其次是醇类,其中γ-松油烯、桃金娘醛、α-蒎烯、β-蒎烯、β-罗勒烯、对伞花烃等6种物质在益智所有组织中均为主要成分;与其他3种组织相比,果实中α-松油烯、桧烯、α-蛇麻烯和反式-β-萜品醇相对含量较高,α-香柑油烯、α-姜黄烯、β-倍半水芹烯等10种化合物未检出;利用主成分分析将52个指标简化为6个综合指标,主成分综合得分从高到低排序为叶片、茎、块根、果实;根据F值的大小,发现益智果实与其他组织挥发油成分差异较大,其他3种组织间差异较小。

       

      Abstract: The fruit of Alpinia oxyphylla was the main harvested part, while the stalks, leaves and roots were usually not used. In order to make full use of these resources, the fresh leaves, stalks, roots and fresh fruits of Alpinia oxyphylla in Fujian were used as the materials. Then, the volatile oil of different tissues at the same time was extracted by water steam distillation, and the content of volatile oil in each tissue was calculated. The volatile oil components in different tissues of Alpinia oxyphylla were analyzed by head-space injecting method and gas chromatography-mass spectrometry (GC-MS). The results showed that: different tissues of the local Alpinia oxyphylla in Fujian contained volatile oil components, among which the content of volatile oil in root tubers was the highest, reaching 0.22 %, followed by leaves and the least in stems. A total of 52 components were detected from the volatile oil components in different tissues of Alpinia oxyphylla. It was found that the number of olefins was the largest, followed by alcohols. Among them, γ-terpinene, myrtenal, α-pinene, β-pinene, β-ocimene and p-cyinene were all the main components in all the tissues of Alpinia oxyphylla. Compared with the other three tissues, the relative contents of α-terpinene, sabinene, α-humulene and trans-β-terpineol in fruits were higher, while the ten compounds such as α-bergamotene, α-curcumene and β-sesquiphellandrene were not detected. The principal component analysis was used to simplify 52 indicators into 6 aggregative indicators. The comprehensive scores of principal components were ranked from high to low as leaves, stalks, roots and fruits. According to the F value, it was found that the volatile oil components in the fruits of Alpinia oxyphylla were quite different from those in other tissues, while the differences among the other three tissues in Alpinia oxyphylla were small.

       

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