李和平,张树河,林江波,等. 福建本地益智不同组织挥发油成分的GC-MS分析 [J]. 福建农业科技,2024,55(7):38−43. DOI: 10.13651/j.cnki.fjnykj.2024.07.007
    引用本文: 李和平,张树河,林江波,等. 福建本地益智不同组织挥发油成分的GC-MS分析 [J]. 福建农业科技,2024,55(7):38−43. DOI: 10.13651/j.cnki.fjnykj.2024.07.007
    LI He-ping, ZHANG Shu-he, LIN Jiang-bo, DAI Yi-min. Analysis of volatile components in different tissues of Fujian Alpinia oxyphylla by GC-MS[J]. Fujian Agricultural Science and Technology. DOI: 10.13651/j.cnki.fjnykj.2024.07.007
    Citation: LI He-ping, ZHANG Shu-he, LIN Jiang-bo, DAI Yi-min. Analysis of volatile components in different tissues of Fujian Alpinia oxyphylla by GC-MS[J]. Fujian Agricultural Science and Technology. DOI: 10.13651/j.cnki.fjnykj.2024.07.007

    福建本地益智不同组织挥发油成分的GC-MS分析

    Analysis of volatile components in different tissues of Fujian Alpinia oxyphylla by GC-MS

    • 摘要: 果实是益智的主要收获部位,而茎秆、叶片和块根通常都未被利用。为了充分利用这些资源,以福建本地益智鲜叶、茎秆、块根和鲜果为材料,采用水蒸气蒸馏法萃取其同一时间不同组织的挥发油,计算各组织挥发油的含量,并采用顶空进样法和气相色谱-质谱联用(GC-MS)技术对益智不同组织中挥发油成分进行分析。结果表明:福建本地益智不同组织均含有挥发油成分,其中块根中挥发油含量最高,达0.22%,其次是叶片,茎最少。益智不同组织挥发油成分共检出52种成分,发现烯烃类物质数量最多,其次是醇类,其中γ-松油烯、桃金娘醛、α-蒎烯、β-蒎烯、β-罗勒烯、对伞花烃等6种物质在益智所有组织中均为主要成分;与其他3种组织相比,果实中α-松油烯、桧烯、α-蛇麻烯和反式-β-萜品醇相对含量较高,α-香柑油烯、α-姜黄烯、β-倍半水芹烯等10种化合物未检出;利用主成分分析将52个指标简化为6个综合指标,主成分综合得分从高到低排序为叶片、茎、块根、果实;根据F值的大小,发现益智果实与其他组织挥发油成分差异较大,其他3种组织间差异较小。

       

      Abstract: The fruit of A. oxyphylla was the main harvested part, while the stalks, leaves and roots were usually left unused. In order to make full use of these tissues with high biological yield, the volatile oil components of different tissues were analyzed by headspace sampling and GC-MS technology in this study, providing a scientific basis for the comprehensive utilization of A. oxyphylla. The results showed that the volatile oil content was the highest in root tuber(0.22%), followed by leaves, and the least in stem. A total of 52 volatile components were detected by GC-MS. Olefin compounds were the most abundant, followed by alcohols. Among them, γ-terpinene, myrtenal, α-pinene, β-pinene, β-Ocimene and p-Cymene were the main components in all tissues of A. oxyphylla. The contents of α-terpinene, sabinene, α-Humulene and trans-β-Terpineol were higher than those of the other 3 tissues, but 10 compounds such as α-bergamotene, α-Curcumene and β-sesquiphellandrene were not detected in fruit. Principal component analysis was used to simplify 52 indexes into 6 composite indexes, and the composite scores of principal components were ranked from high to low as leaves, stems, roots and fruits. According to the size of F value, it was found that the essential oil composition of fruit was different from other tissues, but the difference between the other three tissues was small in A. oxyphylla.

       

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