Abstract: The techniques of artificial seedling raising and juvenile shellfish cultivation of Paphia amabilis were discussed in order to provide technical data for large-scale and efficient breeding and production of P.amabilis seedlings Clustering single factor variance was used to study the key techniques of species identification and seedling breeding of P.amabilis.The results showed that the similarity between the COI sequences of the juvenile shellfish and those of P.amabilis was 100%. All the juvenile shellfish were P.amabilis.The parent shellfish with average shell length of 45.37 mm were selected to induce the fertilized eggs to spawn with dry shade and ammonia seawater. The hatching rate was 59.25% and 91.68%, respectively. Under the condition of water temperature 28.1℃ and salinity 27.5, fertilized eggs developed into D-shaped larvae after 20 hours, and the larvae were developed to larvae after 9 days with cultivate density of 1～2 inds./mL. After 3～5 days cultivation with fine sand as attachment base, all the larvae were developed into juvenile shellfish, and the development rate was over 50%. In the early stage, the shell length of juvenile shellfish cultured with sand was more than 1mm, while the shell length of juvenile shellfish cultured without sand was more than 12 mm after 6 months in indoor cement pond. Juvenile shellfish with shell length was more than 12 mm were used to cultivate medium seedlings in cement pond. The results showed that the growth rate and survival rate of the overhead group were significantly higher than those of the non-overhead group.